Li Rugang, Gao Shan, Fei Zhangjun, Ling Kai-Shu
USDA-Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, South Carolina, USA.
Genome Announc. 2013 Oct 3;1(5):e00794-13. doi: 10.1128/genomeA.00794-13.
The complete genomic sequence of a new tobamovirus in tomatoes was determined through deep sequencing and assembly of small RNAs, then validated through Sanger sequencing. Based on the low sequence identity (≤85%) to known viruses and a close phylogenetic relationship to tobamoviruses, it was identified as a new species.
通过对小RNA进行深度测序和组装,确定了番茄中一种新型烟草花叶病毒的完整基因组序列,然后通过桑格测序进行验证。基于与已知病毒的低序列同一性(≤85%)以及与烟草花叶病毒的密切系统发育关系,它被鉴定为一个新物种。