State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Int J Mol Sci. 2024 Apr 4;25(7):4012. doi: 10.3390/ijms25074012.
(ToBRFV) is a newly-emerging tobamovirus which was first reported on tomatoes in Israel and Jordan, and which has now spread rapidly in Asia, Europe, North America, and Africa. ToBRFV can overcome the resistance to other tobamoviruses conferred by tomato , , and genes, and it has seriously affected global crop production. The rapid and comprehensive transcription reprogramming of host plant cells is the key to resisting virus attack, but there have been no studies of the transcriptome changes induced by ToBRFV in tomatoes. Here, we made a comparative transcriptome analysis between tomato leaves infected with ToBRFV for 21 days and those mock-inoculated as controls. A total of 522 differentially expressed genes were identified after ToBRFV infection, of which 270 were up-regulated and 252 were down-regulated. Functional analysis showed that DEGs were involved in biological processes such as response to wounding, response to stress, protein folding, and defense response. Ten DEGs were selected and verified by qRT-PCR, confirming the reliability of the high-throughput sequencing data. These results provide candidate genes or signal pathways for the response of tomato leaves to ToBRFV infection.
（ToBRFV）是一种新出现的番茄斑驳病毒，最初在以色列和约旦的番茄上报道，现已迅速传播到亚洲、欧洲、北美和非洲。ToBRFV 可以克服番茄、和基因赋予的对其他烟草花叶病毒的抗性，严重影响了全球作物生产。宿主植物细胞的快速和全面转录重编程是抵御病毒攻击的关键，但目前还没有研究番茄感染 ToBRFV 后转录组的变化。在这里，我们对感染 ToBRFV 21 天的番茄叶片和模拟接种的对照叶片进行了比较转录组分析。在 ToBRFV 感染后共鉴定出 522 个差异表达基因，其中 270 个上调，252 个下调。功能分析表明，DEGs 参与了对创伤的反应、对压力的反应、蛋白质折叠和防御反应等生物学过程。选择了 10 个 DEGs 并通过 qRT-PCR 进行了验证，证实了高通量测序数据的可靠性。这些结果为番茄叶片对 ToBRFV 感染的反应提供了候选基因或信号通路。
