Department of Cardiology, Shanghai Ninth People's Hospital Affiliated Shanghai Jiaotong University School of Medicine , Shanghai, China .
Zebrafish. 2014 Feb;11(1):71-5. doi: 10.1089/zeb.2012.0858. Epub 2013 Oct 4.
As zebrafish became a popular research system in contemporary biomedical research, effective anesthesia, which had low toxicity and high efficacy, was needed. The objective of this article was to evaluate the anesthetic effect of rapid cooling for embryo and larvae zebrafish with ice slush (ice and water admixture). The time to stage 5 anesthesia and maintaining for more than 5 s were detected and compared to MS-222 anesthesia. Besides, the time of recovery from anesthesia, mortality, and the survivability were measured and compared with MS-222 anesthesia. The results showed that anesthesia was generally achieved within 10 s for rapid cooling, which was more rapid than MS-222. The survivability assay demonstrated that rapid cooling was suitable for embryo and larvae zebrafish (1-14 days) and could be used for repeated anesthesia. The most important advantage was that this anesthesia could persist for 10 min and had no mortality. These findings suggested that rapid cooling provided advantages of improved safety, rapid anesthesia, and potentially low mortality rates and could be an effective anesthetic method for scientific research.
随着斑马鱼成为当代生物医学研究中一种流行的研究系统，需要一种具有低毒性和高效性的有效麻醉剂。本文旨在评估冰沙（冰和水混合物）快速冷却对胚胎和幼体斑马鱼的麻醉效果。检测并比较了达到 5 期麻醉和维持超过 5 秒的时间与 MS-222 麻醉的效果。此外，还测量和比较了从麻醉中恢复的时间、死亡率和存活率与 MS-222 麻醉的效果。结果表明，快速冷却通常在 10 秒内实现麻醉，比 MS-222 更快。生存能力检测表明，快速冷却适用于胚胎和幼体斑马鱼（1-14 天），可用于重复麻醉。最重要的优点是这种麻醉可以持续 10 分钟，且没有死亡率。这些发现表明，快速冷却提供了提高安全性、快速麻醉以及潜在低死亡率的优势，可能成为科学研究的一种有效麻醉方法。
