从印度奥里萨邦塔塔帕尼温泉分离的产胞外L-天冬酰胺酶枯草芽孢杆菌菌株hswx88的筛选与鉴定
Screening and characterization of extracelluar L-asparaginase producing Bacillus subtilis strain hswx88, isolated from Taptapani hotspring of Odisha, India.
作者信息
Pradhan Biswaprakash, Dash Sashi K, Sahoo Sabuj
机构信息
Pharmaceutical Biotechnology Division, University Department of Pharmaceutical Sciences, Utkal University, Vani Vihar, Bhubaneswar, Odisha-751 004, India.
出版信息
Asian Pac J Trop Biomed. 2013 Dec;3(12):936-41. doi: 10.1016/S2221-1691(13)60182-3.
OBJECTIVE
To screen and isolate an eco-friendly, a thermophilic and potent L-asparaginase producing bacterium, with novel immunological properties that may obviates hypersensitivity reactions.
METHODS
In the present study bacterial strain isolated for extracellular L-asparaginase production from hotspring, identified by morphological, biochemical and physiological tests followed by 16S rDNA technology and the L-asparaginase production ability was tested by both semi quantitative and quantitative enzymatic assay.
RESULTS
The bacterial strain was identified as Bacillus subtilis strain hswx88 (GenBank Accession Number: JQ237656.1). The extracellular enzyme yielding capacity isolate Bacillus subtilis strain hswx88 (23.8 IU/mL) was found to be 1.7 and 14.5 times higher than the reference organism Pectobacterium carotovorum MTCC 1428 (14.2 IU/mL) and Bacillus sp. BCCS 034 (1.64 IU/mL).
CONCLUSION
The isolate is eco-friendly and useful to produce bulk quantity of extracellular, thermophilic L-asparaginase for the treatment of various tumor cases and for preparation of acrylamide free fry food preparation.
目的
筛选并分离出一种生态友好、嗜热且高效产L-天冬酰胺酶的细菌，该细菌具有可避免过敏反应的新型免疫学特性。
方法
在本研究中，从温泉中分离出用于胞外L-天冬酰胺酶生产的细菌菌株，通过形态学、生化和生理学测试以及16S rDNA技术进行鉴定，并通过半定量和定量酶测定法测试L-天冬酰胺酶的生产能力。
结果
该细菌菌株被鉴定为枯草芽孢杆菌菌株hswx88（GenBank登录号：JQ237656.1）。发现枯草芽孢杆菌菌株hswx88的胞外酶产量（23.8 IU/mL）分别比参考菌株胡萝卜软腐果胶杆菌MTCC 1428（14.2 IU/mL）和芽孢杆菌属BCCS 034（1.64 IU/mL）高1.7倍和14.5倍。
结论
该分离株生态友好，可用于大量生产胞外嗜热L-天冬酰胺酶，用于治疗各种肿瘤病例以及制备无丙烯酰胺的油炸食品。
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