A new technique for differential and simultaneous staining of nerve cells and fibers.

A new and simple method for the simultaneous staining of nerve cells and fibers is described. The use of formol-thionin under controlled conditions of concentration and pH provides a highly reliable method for the staining of nerve cells in blue and fiber tracts in red, making any posterior differentiation redundant. This technique provides constant and reproducible results allowing simultaneous staining of many sections.