Beijing Key Laboratory for Solid Waste Utilization and Management, College of Engineering, Peking University, Beijing 100871, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Department of Burns, Beijing Jishuitan Hospital, Beijing 100035, China.
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4816-24. doi: 10.1016/j.msec.2013.07.044. Epub 2013 Aug 7.
A series of carboxymethyl chitosan (CM-chitosan) and gelatin hydrogels were prepared by radiation crosslinking. A pre-clinical study was performed by implantation model and full-thickness cutaneous wound model in Sprague-Dawley rats to preliminarily evaluate the biocompatibility, biodegradability and effects on healing. In the implantation test, as a component of the hydrogels, CM-chitosan showed a positive effect on promoting cell proliferation and neovascularization, while gelatin was efficient to stabilize the structure and prolong the degradation time. To evaluate the function on wound healing, the hydrogels were applied to the relatively large full-thickness cutaneous wounds (Φ3.0 cm). Compared with the control groups, the hydrogel group showed significantly higher percentage of wound closure on days 9, 12 and 15 postoperatively, which was consistent with the significantly thicker granulation tissue on days 3 and 6. All results apparently revealed that the radiation crosslinked CM-chitosan/Gelatin hydrogels could induce granulation tissue formation and accelerate the wound healing.
采用辐射交联法制备了一系列羧甲基壳聚糖（CM-壳聚糖）和明胶水凝胶。通过植入模型和 Sprague-Dawley 大鼠全层皮肤创面模型进行了临床前研究，初步评价了其生物相容性、可生物降解性和对愈合的影响。在植入试验中，CM-壳聚糖作为水凝胶的组成部分，对促进细胞增殖和新生血管形成有积极作用，而明胶则能有效稳定结构并延长降解时间。为了评估对创面愈合的作用，将水凝胶应用于相对较大的全层皮肤创面（Φ3.0cm）。与对照组相比，水凝胶组在术后第 9、12 和 15 天的创面闭合率显著提高，这与第 3 和 6 天肉芽组织明显增厚的结果一致。所有结果均明显表明，辐射交联的 CM-壳聚糖/明胶水凝胶可诱导肉芽组织形成并加速创面愈合。
