School of Chemistry and Biochemistry, The University of Western Australia (M313), Crawley, WA 6009, Australia.
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4917-22. doi: 10.1016/j.msec.2013.08.011. Epub 2013 Aug 17.
A monomer that contained the RGD ligand motif was synthesized and copolymerized with 2-hydroxyethyl methacrylate using polymerization-induced phase separation methods to form poly(2-hydroxyethyl methacrylate)-based hydrogel sponges. The sponges had morphologies of aggregated polymer droplets and interconnected pores, the pores having dimensions in the order of 10 μm typical of PHEMA sponges. RGD-containing moieties appeared to be evenly distributed through the polymer droplets. Compared to PHEMA sponges that were not functionalized with RGD, the new sponges containing RGD allowed greater invasion by human corneal epithelial cells, by advancing the attachment of cells to the surface of the polymer droplets.
合成了一种含有 RGD 配体基序的单体，并通过聚合诱导相分离方法与 2-羟乙基甲基丙烯酸酯共聚，形成基于聚（2-羟乙基甲基丙烯酸酯）的水凝胶海绵。海绵具有聚合物液滴聚集和相互连接的孔的形态，孔的尺寸为 10μm 左右，与 PHEMA 海绵典型尺寸相当。含有 RGD 的部分似乎均匀分布在聚合物液滴中。与未用 RGD 功能化的 PHEMA 海绵相比，新的含有 RGD 的海绵允许人角膜上皮细胞更大程度地侵入，通过促进细胞附着在聚合物液滴表面来实现。
