Verwaerde C, Auriault C, Damonneville M, Grzych J M, Pierce R, Capron A
Parasitology. 1985 Jun;90 ( Pt 3):509-18. doi: 10.1017/s0031182000055505.
The participation of products released from Schistosoma mansoni schistosomula (SRP-A) in the IgG antibody response of infected Brown-Norway rats and infected humans has been studied using immunoprecipitation with various antigenic preparations and in in vitro cytotoxicity assays. A large number of SRP-A molecules with a wide range of molecular weights was recognized by infected rat and human sera. Anti-SRP-A antibodies appeared in rat sera from day 28 after infection. In infected humans, a variable pattern of SRP-A recognition was observed between individuals. IgG antibodies obtained by immunization of rats with SRP-A without addition of adjuvants reacted with 3 major schistosomula surface proteins with molecular weights of 38, 32 and 21 kDa. These latter molecules were also revealed strongly by infected rat sera. Moreover, these antibodies were able to kill schistosomula in vitro in the presence of complement or eosinophils.
利用各种抗原制剂进行免疫沉淀以及体外细胞毒性试验，研究了曼氏血吸虫童虫释放产物（SRP-A）在受感染的Brown-Norway大鼠和受感染人类的IgG抗体反应中的参与情况。受感染大鼠和人类血清识别出大量分子量范围广泛的SRP-A分子。感染后第28天，大鼠血清中出现抗SRP-A抗体。在受感染的人类中，个体之间观察到SRP-A识别的可变模式。用未添加佐剂的SRP-A免疫大鼠获得的IgG抗体与分子量分别为38、32和21 kDa的3种主要童虫表面蛋白发生反应。受感染大鼠的血清也强烈显示出这些后者分子。此外，这些抗体在补体或嗜酸性粒细胞存在的情况下能够在体外杀死童虫。
