Auriault C, Damonneville M, Joseph M, Capron M, Verwaerde C, Billaut P, Capron A
Eur J Immunol. 1985 Dec;15(12):1168-72. doi: 10.1002/eji.1830151205.
The study of the immunology of schistosomiasis has allowed a clear understanding of the basic mechanisms of resistance, emphasizing the important role played by cellular and humoral factors. Whereas the production of polyclonal or monoclonal antibodies and the precise inventory of immune effector mechanisms in the rat and in man have led to the identification of potentially protective antigens, immunization with soluble schistosome components has not allowed a successful control of the destruction of schistosomula after infection. The experiments reported here show that schistosomulum-released products (SRP) were able to induce the production of antibodies, in the rat and the monkey, highly cytotoxic in antibody-dependent cellular cytotoxicity, using monocyte monolayers, platelets or eosinophils as effector cells. The immunization of rats with either total SRP or 25-30-kDa molecules purified from schistosomula conferred a significant protection towards a challenge infection by the parasite. IgE and to a lesser extent IgG antibodies represented the major humoral factors of cell activation leading to the schistosomulum killing when anti-SRP antisera, obtained after immunization of the monkey, were incubated with human effector cells.
血吸虫病免疫学的研究使人们清楚地了解了抵抗的基本机制，强调了细胞和体液因素所起的重要作用。虽然多克隆或单克隆抗体的产生以及对大鼠和人类免疫效应机制的精确梳理已导致鉴定出潜在的保护性抗原，但用可溶性血吸虫成分进行免疫并未成功控制感染后血吸虫幼虫的破坏。此处报道的实验表明，血吸虫幼虫释放产物（SRP）能够在大鼠和猴子体内诱导抗体产生，这些抗体在抗体依赖的细胞毒性作用中具有高度细胞毒性，使用单核细胞单层、血小板或嗜酸性粒细胞作为效应细胞。用总SRP或从血吸虫幼虫中纯化的25 - 30 kDa分子对大鼠进行免疫，可对寄生虫的攻击感染提供显著保护。当用猴子免疫后获得的抗SRP抗血清与人效应细胞孵育时，IgE以及程度稍低的IgG抗体是导致血吸虫幼虫杀伤的细胞活化的主要体液因素。
