家禽精液的荧光测定法：其在活力、酶泄漏及受精率测定中的应用
Fluorometry of poultry semen: its application in the determination of viability, enzyme leakage, and fertility.
作者信息
Bilgili S F, Renden J A, Sexton K J
出版信息
Poult Sci. 1985 Jun;64(6):1227-30. doi: 10.3382/ps.0641227.
The accuracy of fluorometry for estimating percentages of dead chicken spermatozoa was investigated by comparing this technique with the eosin-nigrosin differential staining procedure and with glutamic oxaloacetic transaminase (GOT) concentration in seminal plasma. The relationship between percent dead sperm measured by fluorometry and fertility was also examined. The correlation coefficient of percentage of dead spermatozoa determined by fluorometry with eosin-nigrosin counts was highly significant (r = .99; P less than .001). Similarly, the correlation coefficient of GOT activity with percentage of dead spermatozoa was .99 (P less than .001). Percent fertility, fertile egg production, and duration of fertility were negatively correlated with percent dead spermatozoa; 4 = -.55, -.51, and -.44 (P less than .001), respectively.
通过将荧光测定法与伊红 - 黑色素差异染色法以及精浆中谷草转氨酶（GOT）浓度进行比较，研究了荧光测定法估计死鸡精子百分比的准确性。还检测了通过荧光测定法测得的死精子百分比与受精率之间的关系。荧光测定法测定的死精子百分比与伊红 - 黑色素计数的相关系数非常显著（r = 0.99；P < 0.001）。同样，GOT活性与死精子百分比的相关系数为0.99（P < 0.001）。受精率、可孵化蛋产量和受精持续时间与死精子百分比呈负相关；分别为r = -0.55、-0.51和-0.44（P < 0.001）。
Zotero 插件