Bilgili S F, Sexton K J, Renden J A
Poultry Science Department, Alabama Agricultural Experiment Station, Auburn University 36849.
Poult Sci. 1987 Dec;66(12):2032-5. doi: 10.3382/ps.0662032.
Two experiments were conducted to measure the effects of semen dilution and storage time (0, 1, 2, 3, 4, 24, and 48 h) at 22 C on spermatozoal viability (i.e., membrane permeability to ethidium bromide) and to determine the relationship between concentration of viable spermatozoa inseminated (25, 50, 100, and 200 x 10(6] and fertility. In Experiment 1, percentages of dead spermatozoa remained relatively constant during the 4-h postcollection period but increased significantly (P less than .05) at 24 and 48 h. Sperm viability after 48 h was significantly higher in diluted semen than in undiluted semen. Percent (PF) and duration of fertility (DF) from undiluted semen significantly declined during the 4-h postcollection period compared with fertility of diluted semen. In Experiment 2, both PF and DF improved as the concentration of viable spermatozoa increased. Fertility was not significantly improved by inseminating more than 100 X 10(6) viable spermatozoa. The fertilizing capacity of chicken spermatozoa from undilated semen was affected during storage before membrane permeability to ethidium bromide was altered.
进行了两项试验，以测定精液稀释和在22℃下储存时间（0、1、2、3、4、24和48小时）对精子活力（即对溴化乙锭的膜通透性）的影响，并确定授精时存活精子浓度（25、50、100和200×10⁶）与受精率之间的关系。在试验1中，采精后4小时内死精子百分比保持相对稳定，但在24和48小时时显著增加（P<0.05）。48小时后，稀释精液中的精子活力显著高于未稀释精液。与稀释精液的受精率相比，未稀释精液的受精百分比（PF）和受精持续时间（DF）在采精后4小时内显著下降。在试验2中，PF和DF均随存活精子浓度的增加而提高。授精超过100×10⁶个存活精子时，受精率没有显著提高。未稀释精液中鸡精子的受精能力在储存期间受到影响，而此时对溴化乙锭的膜通透性尚未改变。
