Department of Genetics and Molecular Biology, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, China.
Physiol Behav. 2014 Jan 17;123:105-13. doi: 10.1016/j.physbeh.2013.09.010. Epub 2013 Oct 2.
Most existing animal models for stress involve the simultaneous application of physical and psychological stress factors. In the current study, we described and used a novel psychological stress model (scream sound stress). To study the validity of it, we carried out acute and chronic scream sound stress. First, adult Sprague-Dawley (SD) rats were randomly divided into white noise, stress and background groups. The white noise group and stress group were treated with white noise and scream sound for 4h in the morning respectively. Compared with white noise and background groups, exposure to acute scream sound increased corticosterone (CORT) level and decreased latency in Morris water maze (MWM) test. The levels of noradrenaline (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were altered in the striatum, hypothalamus and hippocampus of stress rats. Second, adult SD rats were randomly divided into background and stress groups, which were treated with scream sound for three weeks. Exposure to chronic scream sound suppressed body weight gain, increased corticosterone (CORT) level, influenced the morphology of adrenal gland, improved spleen and thymus indices, and decreased latency in MWM test. NE, DA, DOPAC, HVA and 5-HIAA levels were also altered in the brain of stress rats. Our results suggested that scream sound, as a novel stressor, facilitated learning ability, as well as altered monoamine levels in the rat brain. Moreover, scream sound is easy to apply and can be applied in more animals at the same time.
大多数现有的应激动物模型都涉及到生理和心理应激因素的同时应用。在本研究中，我们描述并使用了一种新的心理应激模型（尖叫应激）。为了研究其有效性，我们进行了急性和慢性尖叫应激。首先，成年 Sprague-Dawley（SD）大鼠随机分为白噪声组、应激组和背景组。白噪声组和应激组分别于上午接受白噪声和尖叫声处理 4 小时。与白噪声和背景组相比，急性尖叫暴露增加了皮质酮（CORT）水平，降低了 Morris 水迷宫（MWM）测试中的潜伏期。应激大鼠纹状体、下丘脑和海马中的去甲肾上腺素（NE）、多巴胺（DA）、5-羟色胺（5-HT）、3,4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）和 5-羟吲哚乙酸（5-HIAA）水平发生改变。其次，成年 SD 大鼠随机分为背景组和应激组，接受尖叫声处理 3 周。慢性尖叫暴露抑制了体重增加，增加了皮质酮（CORT）水平，影响了肾上腺形态，提高了脾和胸腺指数，并降低了 MWM 测试中的潜伏期。应激大鼠大脑中的 NE、DA、DOPAC、HVA 和 5-HIAA 水平也发生了改变。我们的结果表明，尖叫作为一种新的应激源，促进了学习能力，并改变了大鼠大脑中的单胺水平。此外，尖叫很容易应用，可以同时应用于更多的动物。
