Division of Plant Biosciences, School of Applied BioSciences, College of Agriculture and Life Science, Kyungpook National University, Taegu, 702-701, Korea.
Mol Biol Rep. 2013 Nov;40(11):6113-21. doi: 10.1007/s11033-013-2723-z. Epub 2013 Oct 5.
We identified rice genes that might be involved in drought stress tolerance by virtue of their anti-apoptotic activity. Potential anti-apoptosis related genes were identified by screening an Oryza sativa cDNA library derived from drought stressed tissues in a yeast functional assay. About 28 O. sativa cDNAs promoted yeast survival following engagement of Bax-induced apoptosis. An O. sativa cDNA encoding R12H780 was a highly conserved putative senescence-associated-protein (OsSAP). OsSAP was both highly and rapidly expressed in response to drought stress. Additionally, OsSAP was found to be localized to the mitochondria. Overall, OsSAP represents a new type of Bax suppressor related gene and endows multiple stress tolerance in yeast.
我们通过其抗细胞凋亡活性鉴定出可能参与干旱胁迫耐受的水稻基因。通过酵母功能测定筛选来自干旱胁迫组织的水稻 cDNA 文库，鉴定出潜在的抗细胞凋亡相关基因。约 28 个水稻 cDNA 在 Bax 诱导的细胞凋亡作用下促进酵母存活。编码 R12H780 的水稻 cDNA 是一个高度保守的假定衰老相关蛋白（OsSAP）。OsSAP 对干旱胁迫的响应既高度又迅速。此外，发现 OsSAP 定位于线粒体。总的来说，OsSAP 代表了一种新型的 Bax 抑制相关基因，并赋予酵母多种胁迫耐受性。
