Nosheen Asia, Bano Asghari, Ullah Faizan
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Toxicol Ind Health. 2016 Feb;32(2):270-7. doi: 10.1177/0748233713498453. Epub 2013 Oct 4.
This study aimed to find out the effect of plant growth-promoting rhizobacteria (PGPR; Azospirillum brasilense and Azotobacter vinelandii) either alone or in combination with different doses of nitrogen and phosphate fertilizers on growth, seed yield, and oil quality of Brassica carinata (L.) cv. Peela Raya. PGPR were applied as seed inoculation at 10(6) cells/mL(-1) so that the number of bacterial cells per seed was 2.6 × 10(5) cells/seed. The chemical fertilizers, namely, urea and diammonium phosphate (DAP) were applied in different doses (full dose (urea 160 kg ha(-1) + DAP 180 kg ha(-1)), half dose (urea 80 kg ha(-1) + DAP 90 kg ha(-1)), and quarter dose (urea 40 kg ha(-1) + DAP 45 kg ha(-1)). The chemical fertilizers at full and half dose significantly increased the chlorophyll, carotenoids, and protein content of leaves and the seed yield (in kilogram per hectare) but had no effect on the oil content of seed. The erucic acid (C22:1) content present in the seed was increased. Azospirillum performed better than Azotobacter and its effect was at par with full dose of chemical fertilizers (CFF) for pigments and protein content of leaves when inoculated in the presence of half dose of chemical fertilizers (SPH). The seed yield and seed size were greater. Supplementing Azospirillum with SPH assisted Azospirillum to augment the growth and yield, reduced the erucic acid (C22:1) and glucosinolates contents, and increased the unsaturation in seed oil. It is inferred that A. brasilense could be applied as an efficient bioinoculant for enhancing the growth, seed yield, and oil quality of Ethiopian mustard at low fertilizer costs and sustainable ways.
本研究旨在探究促生根际细菌（PGPR，即巴西固氮螺菌和维涅兰德固氮菌）单独或与不同剂量的氮磷肥组合施用时，对埃塞俄比亚芥（Brassica carinata (L.) cv. Peela Raya）生长、种子产量和油品质的影响。PGPR以10(6)个细胞/毫升(-1)的浓度进行种子接种，使每粒种子的细菌细胞数为2.6×10(5)个细胞/种子。化学肥料，即尿素和磷酸二铵（DAP），以不同剂量施用（全量（尿素160千克/公顷 + DAP 180千克/公顷）、半量（尿素80千克/公顷 + DAP 90千克/公顷）和四分之一量（尿素40千克/公顷 + DAP 45千克/公顷））。全量和半量的化学肥料显著提高了叶片的叶绿素、类胡萝卜素和蛋白质含量以及种子产量（千克/公顷），但对种子的油含量没有影响。种子中的芥酸（C22:1）含量增加。巴西固氮螺菌的效果优于维涅兰德固氮菌，当与半量化学肥料（SPH）一起接种时，其对叶片色素和蛋白质含量的影响与全量化学肥料（CFF）相当。种子产量和种子大小更大。用SPH补充巴西固氮螺菌有助于其促进生长和提高产量，降低芥酸（C22:1）和硫代葡萄糖苷含量，并增加种子油的不饱和度。据推断，巴西固氮螺菌可以作为一种有效的生物接种剂，以低成本和可持续的方式提高埃塞俄比亚芥的生长、种子产量和油品质。
