Koyu Ahmet, Gokalp Osman, Gumral Nurhan, Oktem Faruk, Karahan Nermin, Yilmaz Nigar, Saygin Mustafa
Department of Physiology, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey
Department of Pharmacology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
Toxicol Ind Health. 2016 Feb;32(2):306-12. doi: 10.1177/0748233713501708. Epub 2013 Oct 4.
This study investigates the preventive effect of caffeic acid phenethyl ester (CAPE) on pancreatic damage induced by vancomycin (VCM) in rats. Rats were equally divided into three groups: group I (control), group II (only VCM-treated group) and group III (VCM + CAPE-treated groups). VCM was intraperitoneally administered at a dose of 200 mg kg(-1)twice daily for 7 days. CAPE was administered orally at 10 µM mL(-1) kg(-1) dose once daily for 7 days. The first dose of CAPE administration was performed 24 h prior to VCM injection. Blood and pancreas tissue samples were removed and collected after the study. Serum alkaline phosphatase (ALP), amylase, γ-glutamyl transferase (GGT) and lipase activities were determined. Pancreas tissue samples were evaluated with the light microscope. Group II significantly increased serum ALP, amylase, GGT and lipase activities when compared with the control group. Group III significantly decreased serum ALP, amylase, GGT and lipase activities when compared with group II. In histopathological examination, it has been observed that there was a significant pancreatic damage in group II. CAPE exerted prominent structural protection against VCM-induced pancreatic damage and this effect was statistically significant. CAPE caused a marked reduction in the extent of pancreatic damage. We have concluded that it may play an important role in the VCM-induced pancreatic damage and reduce the pancreatic damage both at the biochemical and histopathological aspects.
本研究调查了咖啡酸苯乙酯（CAPE）对万古霉素（VCM）诱导的大鼠胰腺损伤的预防作用。大鼠被平均分为三组：第一组（对照组）、第二组（仅用VCM处理组）和第三组（VCM + CAPE处理组）。VCM以200 mg kg⁻¹的剂量腹腔注射，每日两次，共7天。CAPE以10 µM mL⁻¹ kg⁻¹的剂量口服，每日一次，共7天。CAPE的首次给药在VCM注射前24小时进行。研究结束后采集血液和胰腺组织样本。测定血清碱性磷酸酶（ALP）、淀粉酶、γ-谷氨酰转移酶（GGT）和脂肪酶活性。胰腺组织样本用光学显微镜进行评估。与对照组相比，第二组血清ALP、淀粉酶、GGT和脂肪酶活性显著升高。与第二组相比，第三组血清ALP、淀粉酶、GGT和脂肪酶活性显著降低。在组织病理学检查中，观察到第二组存在明显的胰腺损伤。CAPE对VCM诱导的胰腺损伤具有显著的结构保护作用，且这种作用具有统计学意义。CAPE使胰腺损伤程度显著降低。我们得出结论，它可能在VCM诱导的胰腺损伤中起重要作用，并在生化和组织病理学方面减轻胰腺损伤。
