Grotmol T, Buanes T, Mathisen O, Schistad O, Sejersted O M, Raeder M G
Acta Physiol Scand. 1985 May;124(1):71-80. doi: 10.1111/j.1748-1716.1985.tb07633.x.
To study the role played by Na,K-ATPase in the pancreatic secretion of NaHCO3, experiments were performed in 20 anaesthetized, secretin-infused pigs (3.0 clinical units X kg b. wt. X h-I). The relationship between pancreatic NaHCO3 secretion and arterial pH was obtained before and during Na,K-ATPase inhibition by digitoxin and hypokalaemia. Na,K-ATPase activity in pancreatic tissue homogenate averaged 5.45 (5.02-6.68) mumol Pi X mg X protein X h-I. Retrograde injection of 0.5 ml 1.4 X 10(-4) mol X l-I digitoxin into pancreatic ducts reduced pancreatic Na,K-ATPase activity by 3I(I8-47)%, while intra-arterial injection of 0.2 mg X kg b. wt-I digitoxin reduced pancreatic Na,K-ATPase activity by 50(45-56)%. Digitoxin and hypokalaemia reduced the rate of pancreatic NaHCO3 and shifted the normal, proportional relationship between NaHCO3 secretion and arterial pH towards higher pH. Hypokalaemia reduced Na,K-ATPase activity and NaHCO3 secretion in proportion. These effects indicate that Na,K-ATPase helps to sustain the requisite electrochemical potential gradients for driving H+ ions, and hence HCO-3 ions, out of secretory cells.
为研究钠钾ATP酶在胰腺分泌碳酸氢钠中的作用，对20只麻醉后输注促胰液素的猪（3.0临床单位×千克体重×小时⁻¹）进行了实验。在使用洋地黄毒苷抑制钠钾ATP酶和低钾血症之前及期间，获取了胰腺碳酸氢钠分泌与动脉血pH值之间的关系。胰腺组织匀浆中的钠钾ATP酶活性平均为5.45（5.02 - 6.68）微摩尔无机磷×毫克蛋白×小时⁻¹。向胰管逆行注射0.5毫升1.4×10⁻⁴摩尔/升的洋地黄毒苷可使胰腺钠钾ATP酶活性降低31（18 - 47）%，而动脉内注射0.2毫克/千克体重的洋地黄毒苷可使胰腺钠钾ATP酶活性降低50（45 - 56）%。洋地黄毒苷和低钾血症降低了胰腺碳酸氢钠的分泌速率，并使碳酸氢钠分泌与动脉血pH值之间的正常比例关系向更高的pH值偏移。低钾血症按比例降低了钠钾ATP酶活性和碳酸氢钠分泌。这些效应表明，钠钾ATP酶有助于维持驱动氢离子从而驱动碳酸氢根离子排出分泌细胞所需的电化学势梯度。
