通过同源重组修复链断裂。
Repair of strand breaks by homologous recombination.
作者信息
Jasin Maria, Rothstein Rodney
机构信息
Developmental Biology Program, Memorial Sloan-Kettering Cancer Center New York, New York 10065.
出版信息
Cold Spring Harb Perspect Biol. 2013 Nov 1;5(11):a012740. doi: 10.1101/cshperspect.a012740.
Abstract
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.
摘要
在本综述中，我们讨论利用同源DNA序列修复DNA双链断裂（DSB，即同源重组[HR]），主要聚焦于酵母和哺乳动物。我们为当前对同源重组的观点提供了历史背景，并描述了在同源重组过程中双链断裂是如何被处理的，以及与其他双链断裂修复途径的相互作用。我们讨论了该过程的酶学，随后是对活细胞中双链断裂修复的研究。只要有可能，我们会引用原始文章和综述，以帮助读者进行进一步研究。
相似文献
1
Repair of strand breaks by homologous recombination.通过同源重组修复链断裂。
Cold Spring Harb Perspect Biol. 2013 Nov 1;5(11):a012740. doi: 10.1101/cshperspect.a012740.
2
The role of DNA double-strand breaks in spontaneous homologous recombination in S. cerevisiae.DNA双链断裂在酿酒酵母自发同源重组中的作用。
PLoS Genet. 2006 Nov 10;2(11):e194. doi: 10.1371/journal.pgen.0020194. Epub 2006 Oct 5.
3
The role of the Mre11-Rad50-Nbs1 complex in double-strand break repair-facts and myths.Mre11-Rad50-Nbs1复合物在双链断裂修复中的作用——事实与误解
J Radiat Res. 2016 Aug;57 Suppl 1(Suppl 1):i25-i32. doi: 10.1093/jrr/rrw034. Epub 2016 Jun 15.
4
Transcriptionally active chromatin recruits homologous recombination at DNA double-strand breaks.转录活跃的染色质将同源重组募集到 DNA 双链断裂处。
Nat Struct Mol Biol. 2014 Apr;21(4):366-74. doi: 10.1038/nsmb.2796. Epub 2014 Mar 23.
5
Rad51-independent interchromosomal double-strand break repair by gene conversion requires Rad52 but not Rad55, Rad57, or Dmc1.通过基因转换进行的不依赖Rad51的染色体间双链断裂修复需要Rad52，但不需要Rad55、Rad57或Dmc1。
Mol Cell Biol. 2008 Feb;28(3):897-906. doi: 10.1128/MCB.00524-07. Epub 2007 Nov 26.
6
Switching yeast from meiosis to mitosis: double-strand break repair, recombination and synaptonemal complex.酵母从减数分裂转换为有丝分裂：双链断裂修复、重组与联会复合体
Genes Cells. 1997 Aug;2(8):487-98. doi: 10.1046/j.1365-2443.1997.1370335.x.
7
Atmospheric-pressure plasma jet induces DNA double-strand breaks that require a Rad51-mediated homologous recombination for repair in Saccharomyces cerevisiae.大气压等离子体射流诱导DNA双链断裂，在酿酒酵母中这种断裂需要Rad51介导的同源重组来进行修复。
Arch Biochem Biophys. 2014 Oct 15;560:1-9. doi: 10.1016/j.abb.2014.07.029. Epub 2014 Jul 30.
8
Repair of DNA double-strand breaks in plant meiosis: role of eukaryotic RecA recombinases and their modulators.植物减数分裂中DNA双链断裂的修复：真核生物RecA重组酶及其调节剂的作用
Plant Reprod. 2023 Mar;36(1):17-41. doi: 10.1007/s00497-022-00443-6. Epub 2022 Jun 1.
9
Meiotic crossover control by concerted action of Rad51-Dmc1 in homolog template bias and robust homeostatic regulation.同源模板偏向和稳健的内稳态调节中 Rad51-Dmc1 的协同作用对减数分裂交叉控制。
PLoS Genet. 2013;9(12):e1003978. doi: 10.1371/journal.pgen.1003978. Epub 2013 Dec 19.
10
A meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination.一种减数分裂特异性 BRCA2 结合蛋白将重组酶募集到 DNA 双链断裂处以确保同源重组。
Nat Commun. 2019 Feb 13;10(1):722. doi: 10.1038/s41467-019-08676-2.
引用本文的文献
1
Cutting-edge technologies in neural regeneration.神经再生领域的前沿技术。
Cell Regen. 2025 Sep 5;14(1):38. doi: 10.1186/s13619-025-00260-y.
2
The Eukaryotic homology search complex distorts donor DNA structure to probe for homology.真核生物同源性搜索复合体扭曲供体DNA结构以探测同源性。
bioRxiv. 2025 Aug 28:2025.08.28.672940. doi: 10.1101/2025.08.28.672940.
3
Targeting MMR-deficient colorectal cancer with a potent small molecule UNI110.用强效小分子UNI110靶向错配修复缺陷型结直肠癌。
Anim Cells Syst (Seoul). 2025 Aug 4;29(1):502-511. doi: 10.1080/19768354.2025.2542172. eCollection 2025.
4
Molecular tumor boards in pancreatic cancer with liver metastasis: A case report.伴有肝转移的胰腺癌分子肿瘤委员会：一例报告
World J Hepatol. 2025 Jul 27;17(7):106993. doi: 10.4254/wjh.v17.i7.106993.
5
Gene editing and CRISPR-dependent homology-mediated end joining.基因编辑与CRISPR依赖的同源介导的末端连接
Exp Mol Med. 2025 Jul;57(7):1409-1418. doi: 10.1038/s12276-025-01442-z. Epub 2025 Jul 31.
6
Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombination.Rad52和Rad55-Rad57在同源重组中形成Rad51细丝的机制。
Nat Commun. 2025 Jul 21;16(1):6685. doi: 10.1038/s41467-025-61811-0.
7
Comprehensive RAD51C ovarian cancer variant analysis uncouples homologous recombination and replicative functions.RAD51C在卵巢癌中的全面变异分析揭示了同源重组与复制功能的解偶联。
Nat Commun. 2025 Jul 16;16(1):6539. doi: 10.1038/s41467-025-61283-2.
8
Design principle of successful genome editing applications using CRISPR-based toolkits.使用基于CRISPR的工具包成功进行基因组编辑应用的设计原则。
J Appl Genet. 2025 Jul 1. doi: 10.1007/s13353-025-00979-z.
9
Vimentin intermediate filaments orchestrate DNA nonhomologous end joining repair and lipolysis after DNA damage.波形蛋白中间丝在DNA损伤后协调DNA非同源末端连接修复和脂肪分解。
Oncogene. 2025 Jun 23. doi: 10.1038/s41388-025-03465-2.
10
Break-induced replication is activated to repair R-loop-associated double-strand breaks in SETX-deficient cells.在SETX缺陷细胞中，诱导断裂修复被激活以修复与R环相关的双链断裂。
bioRxiv. 2025 May 19:2024.06.29.601361. doi: 10.1101/2024.06.29.601361.
本文引用的文献
1
DNA repair at telomeres: keeping the ends intact.端粒处的 DNA 修复：保持端粒完整。
Cold Spring Harb Perspect Biol. 2013 Jun 1;5(6):a012666. doi: 10.1101/cshperspect.a012666.
2
Repair of double-strand breaks by end joining.非同源末端连接修复双链断裂。
Cold Spring Harb Perspect Biol. 2013 May 1;5(5):a012757. doi: 10.1101/cshperspect.a012757.
3
Numerical constraints and feedback control of double-strand breaks in mouse meiosis.减数分裂中双链断裂的数值限制和反馈控制。
Genes Dev. 2013 Apr 15;27(8):873-86. doi: 10.1101/gad.213652.113. Epub 2013 Apr 18.
4
RIF1 counteracts BRCA1-mediated end resection during DNA repair.RIF1 拮抗 BRCA1 介导的 DNA 修复过程中的末端切除。
J Biol Chem. 2013 Apr 19;288(16):11135-43. doi: 10.1074/jbc.M113.457440. Epub 2013 Mar 13.
5
Efficient genome editing in zebrafish using a CRISPR-Cas system.利用 CRISPR-Cas 系统在斑马鱼中进行高效的基因组编辑。
Nat Biotechnol. 2013 Mar;31(3):227-9. doi: 10.1038/nbt.2501. Epub 2013 Jan 29.
6
A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and BRCA1-CtIP controls DNA repair pathway choice.一个由 53BP1-RIF1 和 BRCA1-CtIP 组成的细胞周期依赖性调控回路控制 DNA 修复途径的选择。
Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.
7
RIF1 is essential for 53BP1-dependent nonhomologous end joining and suppression of DNA double-strand break resection.RIF1 对于 53BP1 依赖性非同源末端连接和抑制 DNA 双链断裂切除是必需的。
Mol Cell. 2013 Mar 7;49(5):858-71. doi: 10.1016/j.molcel.2013.01.002. Epub 2013 Jan 17.
8
Rif1 prevents resection of DNA breaks and promotes immunoglobulin class switching. Rif1 防止 DNA 断裂的切除，并促进免疫球蛋白类别转换。
Science. 2013 Feb 8;339(6120):711-5. doi: 10.1126/science.1230624. Epub 2013 Jan 10.
9
53BP1 regulates DSB repair using Rif1 to control 5' end resection.53BP1 通过 Rif1 控制 5' 端切除来调节 DSB 修复。
Science. 2013 Feb 8;339(6120):700-4. doi: 10.1126/science.1231573. Epub 2013 Jan 10.
10
RNA-guided human genome engineering via Cas9.通过 Cas9 进行 RNA 引导的人类基因组工程。
Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033. Epub 2013 Jan 3.
Zotero 插件