Analysis of RNA structure by ultraviolet crosslinking and denaturation gel electrophoresis.
作者信息
Quarless S A, Cantor C R
出版信息
Anal Biochem. 1985 Jun;147(2):296-300. doi: 10.1016/0003-2697(85)90275-1.
PMID:2409838
Abstract
Electrophoresis in polyacrylamide gels containing both formamide and urea is a high-resolution technique for the analysis of crosslinked RNA species. Combined with a specific crosslinking agent like uv irradiation, it allows a rapid fingerprint of structural differences between RNA forms. The technique reveals significant differences in the pattern of uv crosslinking of free Escherichia coli 16 S ribosomal RNA compared with the RNA in active or inactive 30 S subunits. Ultraviolet photocrosslinks seen only in the 30 S particle are likely to be tertiary structure contacts.
摘要
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