el-Baradi T T, Raué H A, Linnekamp M, Planta R J
FEBS Lett. 1985 Jul 1;186(1):26-30. doi: 10.1016/0014-5793(85)81332-6.
Saccharomyces carlsbergensis 60 S ribosomal subunits were treated with the hetero-bifunctional crosslinking agent 2-iminothiolane and then subjected to mild UV irradiation to introduce protein-rRNA crosslinks. The major crosslinked products were identified as proteins L2, L3, L5, L19 and L23 of which L5 was found to be crosslinked at a 3-5-fold higher efficiency than the other four. Several additional proteins were cross-linked to a detectable but much lower extent.
将卡尔斯伯酵母60S核糖体亚基用异双功能交联剂2-亚氨基硫杂环戊烷处理，然后进行温和的紫外线照射以引入蛋白质-rRNA交联。主要的交联产物被鉴定为蛋白质L2、L3、L5、L19和L23，其中发现L5的交联效率比其他四种高3至5倍。还有几种其他蛋白质也发生了交联，但程度要低得多，仍可检测到。
