Department of Nutrition, Food & Exercise Sciences and ‡Department of Biological Science, Florida State University , Tallahassee, Florida 32306, United States.
J Agric Food Chem. 2013 Nov 13;61(45):10823-33. doi: 10.1021/jf402851k. Epub 2013 Nov 4.
A sandwich enzyme-linked immunosorbent assay (ELISA) using anti-almond soluble protein rabbit polyclonal antibodies as capture antibodies and murine monoclonal antibody 4C10 as the detection antibodies was developed. The assay is specific and sensitive (3-200 ng almond protein/mL) for almond detection. The standardized assay is accurate (<15% CV) and reproducible (intra- and inter assay variability <15% CV). The assay did not register any cross-reactivity with the tested food matrices, suggesting the assay to be almond amandin specific. The assay could detect the presence of declared almond in the tested matched commercial samples. Further, the assay reliably detected the presence of almonds in the laboratory prepared food samples spiked with almond flour.
建立了一种夹心酶联免疫吸附测定（ELISA）法，使用抗杏仁可溶性蛋白兔多克隆抗体作为捕获抗体，鼠单克隆抗体 4C10 作为检测抗体。该测定方法对杏仁的检测具有特异性和灵敏度（3-200ng 杏仁蛋白/ml）。标准化测定方法准确（<15%CV）且可重现（<15%CV）。该测定方法与所测试的食品基质无任何交叉反应性，表明该测定方法是杏仁 amandin 特异性的。该测定方法可以检测到测试的匹配商业样品中杏仁的存在。此外，该测定方法可以可靠地检测出杏仁粉添加到实验室制备的食品样品中杏仁的存在。
