School of Exercise and Nutritional Sciences, San Diego State University, San Diego, CA, USA.
Fujian Hwa Nan Women's College, Fuzhou, Fujian, China.
Methods Mol Biol. 2024;2717:285-304. doi: 10.1007/978-1-0716-3453-0_19.
Enzyme-linked immunosorbent assay (ELISA) is a widely used analytical technique for food allergen detection and quantification. Validating ELISA protocols is important for both assay developers and end users as it ensures method reliability. This chapter describes the protocols for validating the sensitivity, specificity, precision, accuracy, robustness, and ruggedness of an ELISA. Example procedures are also provided for sample preparation, allergen extraction, and ELISA operation.
酶联免疫吸附测定（ELISA）是一种广泛用于食品过敏原检测和定量的分析技术。验证 ELISA 方案对于检测开发人员和最终用户都很重要，因为它可以确保方法的可靠性。本章描述了验证 ELISA 的灵敏度、特异性、精密度、准确度、稳健性和耐用性的方案。还提供了示例程序，用于样品制备、过敏原提取和 ELISA 操作。
