Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
Biochem Biophys Res Commun. 2013 Nov 1;440(4):576-81. doi: 10.1016/j.bbrc.2013.09.106. Epub 2013 Oct 4.
Hydroxytyrosol acetate (Hd-Ac) is a polyphenol that is present in the olive fruit and oil at a concentration similar to that of hydroxytyrosol (Hd). The effects of Hd-Ac on adipogenesis, lipolysis, and glucose consumption in 3T3-L1 cells were investigated. Treatment with Hd-Ac at concentrations of 0-75 μmol/L inhibited 3T3-L1 differentiation and lipid accumulation in a dose-dependent manner. At the same concentration range, no effect on cell viability was observed in the MTT assay. Inhibition of adipogenesis was associated with the downregulation of PPARγ, C/EBPα, SREBP-1c, and their downstream target genes (GLUT4, CD36, and FAS) as revealed by qRT-PCR. On the other hand, Hd-Ac dose dependently activated glycerol release in fully differentiated 3T3-L1 adipocytes, indicating lipolysis. This stimulation of lipolysis was mediated via the activation of hormone-sensitive lipase (HSL) by phosphorylation at Ser563 and Ser660, and the phosphorylation of perilipin. Further investigation of the in vitro activities of this polyphenol showed that Hd-Ac has the capability to increase glucose consumption in 3T3-L1 adipocytes and C2C12 myotubes.
醋酸羟基酪醇（Hd-Ac）是一种多酚，存在于橄榄果实和油中，其浓度与羟基酪醇（Hd）相似。研究了 Hd-Ac 对 3T3-L1 细胞脂肪生成、脂肪分解和葡萄糖消耗的影响。用 0-75μmol/L 的 Hd-Ac 处理以剂量依赖性方式抑制 3T3-L1 分化和脂质积累。在相同的浓度范围内，MTT 测定未观察到对细胞活力的影响。脂肪生成的抑制与 qRT-PCR 显示的 PPARγ、C/EBPα、SREBP-1c 及其下游靶基因（GLUT4、CD36 和 FAS）的下调有关。另一方面，Hd-Ac 以剂量依赖的方式激活完全分化的 3T3-L1 脂肪细胞中的甘油释放，表明脂肪分解。这种脂肪分解的刺激是通过 Ser563 和 Ser660 磷酸化和脂滴蛋白磷酸化激活激素敏感脂肪酶（HSL）介导的。对这种多酚的体外活性的进一步研究表明，Hd-Ac 具有增加 3T3-L1 脂肪细胞和 C2C12 肌管中葡萄糖消耗的能力。
