Carsana A, Furia A, Calabria R, Palmieri M
Biochim Biophys Acta. 1985 Jul 24;825(3):299-302. doi: 10.1016/0167-4781(85)90017-x.
From studies on the in vitro synthesis of the heavily glycosylated pig pancreas ribonuclease (molecular weight of the protein moiety is 13 786, on the basis of the amino acid composition), the following points emerge: (1) the enzyme is synthesized as a precursor having an apparent molecular weight about 7000 higher than that of the mature non-glycosylated protein; (2) the mRNA coding for the enzyme protein consists of about 950 nucleotides.
通过对高度糖基化猪胰腺核糖核酸酶(根据氨基酸组成,蛋白质部分的分子量为13786)的体外合成研究,得出以下几点:(1)该酶作为前体合成,其表观分子量比成熟的非糖基化蛋白高约7000;(2)编码该酶蛋白的mRNA由约950个核苷酸组成。