Carsana A, Confalone E, Palmieri M, Libonati M, Furia A
Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
Nucleic Acids Res. 1988 Jun 24;16(12):5491-502. doi: 10.1093/nar/16.12.5491.
Although pancreatic ribonucleases are extensively studied proteins, little information is available on nucleic acids coding for these enzymes. Here, for the first time, the structure of a gene coding for such an enzyme, the well known bovine pancreatic ribonuclease, is reported. The coding region of this gene is devoid of introns, whereas the 5' untranslated sequence of the pancreatic transcript contains an intron of 735 nucleotides. This intervening sequence is endowed with signals (CAAT and TATA boxes) which might act as regulatory elements. The structural organization of this gene suggests that the sequence coding for the bovine pancreatic ribonuclease might be expressed under the control of two different promoters.
尽管胰腺核糖核酸酶是经过广泛研究的蛋白质，但关于编码这些酶的核酸的信息却很少。在此，首次报道了编码此类酶（著名的牛胰腺核糖核酸酶）的基因结构。该基因的编码区没有内含子，而胰腺转录本的5'非翻译序列包含一个735个核苷酸的内含子。这个间隔序列具有可能作为调控元件的信号（CAAT盒和TATA盒）。该基因的结构组织表明，编码牛胰腺核糖核酸酶的序列可能在两个不同启动子的控制下表达。
