Rodríguez S, Hein S, Yulis R, Delannoy L, Siegmund I, Rodríguez E
Cell Tissue Res. 1985;240(3):649-62. doi: 10.1007/BF00216353.
Reissner's fiber (RF) of the subcommissural organ (SCO), the central canal and its bordering structures, and the filum terminale were investigated in the bovine spinal cord by use of transmission electron microscopy, histochemical methods and light-microscopic immunocytochemistry. The primary antisera were raised against the bovine RF, or the SCO proper. Comparative immunocytochemical studies were also performed on the lumbo-sacral region of the rat, rabbit, dog and pig. At all levels of the bovine spinal cord, RF was strongly immunoreactive with both antisera. From cervical to upper sacral levels of the bovine spinal cord there was an increasing number of ependymal cells immunostainable with both antisera. The free surface of the central canal was covered by a layer of immunoreactive material. At sacral levels small subependymal immunoreactive cells were observed. From all these structures sharing the same immunoreactivity, only RF was stained by the paraldehyde-fuchsin and periodic-acid-Schiff methods. At the ultrastructural level, ependymal cells with numerous protrusions extending into the central canal were seen in the lower lumbar segments, whereas cells displaying signs of secretory activity were principally found in the ependyma of the upper sacral levels. A few cerebrospinal fluid-contacting neurons were observed at all levels of the spinal cord; they were immunostained with an anti-tubulin serum. The lumbo-sacral segments of the dog, rat and rabbit, either fixed by vascular perfusion or in the same manner as the bovine material, did not show any immunoreactive structure other than RF. The possibilities that the immunoreactive ependymal cells might play a secretory or an absorptive role, or be the result of post-mortem events, are discussed.
运用透射电子显微镜、组织化学方法及光学显微镜免疫细胞化学技术，对牛脊髓中连合下器官（SCO）的赖氏纤维（RF）、中央管及其毗邻结构以及终丝进行了研究。制备了针对牛RF或SCO本身的一抗。还对大鼠、兔、狗和猪的腰骶部区域进行了免疫细胞化学比较研究。在牛脊髓的所有节段，RF与两种抗血清均呈强免疫反应性。在牛脊髓从颈段到上骶段，能用两种抗血清免疫染色的室管膜细胞数量逐渐增加。中央管的游离表面覆盖着一层免疫反应性物质。在骶段观察到小的室管膜下免疫反应性细胞。在所有这些具有相同免疫反应性的结构中，只有RF能用高碘酸-希夫法和副醛-品红法染色。在超微结构水平上，在腰下段可见有许多突起延伸至中央管的室管膜细胞，而显示分泌活动迹象的细胞主要见于上骶段的室管膜。在脊髓的所有节段均观察到一些接触脑脊液的神经元；它们用抗微管蛋白血清进行免疫染色。狗、大鼠和兔的腰骶段要么通过血管灌注固定，要么采用与牛材料相同的方式固定，除了RF外未显示任何免疫反应性结构。文中讨论了免疫反应性室管膜细胞可能发挥分泌或吸收作用，或者是死后事件结果的可能性。
