Blois Shauna L, Banerjee Amrita, Wood R Darren, Park Fiona M
Department of Clinical Studies (Blois, Banerjee, Park) and Department of Pathobiology (Wood), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
Can J Vet Res. 2013 Jul;77(3):231-6.
The objective of this study was to describe the results of thromboelastography platelet mapping (TEG-PM) carried out using 2 techniques in 20 healthy dogs. Maximum amplitudes (MA) generated by thrombin (MAthrombin), fibrin (MAfibrin), adenosine diphosphate (ADP) receptor activity (MAADP), and thromboxane A2 (TxA2) receptor activity (stimulated by arachidonic acid, MAAA) were recorded. Thromboelastography platelet mapping was carried out according to the manufacturer's guidelines (2-analyzer technique) and using a variation of this method employing only 1 analyzer (1-analyzer technique) on 2 separate blood samples obtained from each dog. Mean [± standard deviation (SD)] MA values for the 1-analyzer/2-analyzer techniques were: MAthrombin = 51.9 mm (± 7.1)/52.5 mm (± 8.0); MAfibrin = 20.7 mm (± 21.8)/23.0 mm (± 26.1); MAADP = 44.5 mm (± 15.6)/45.6 mm (± 17.0); and MAAA = 45.7 mm (± 11.6)/45.0 mm (± 15.4). Mean (± SD) percentage aggregation due to ADP receptor activity was 70.4% (± 32.8)/67.6% (± 33.7). Mean percentage aggregation due to TxA2 receptor activity was 77.3% (± 31.6)/78.1% (± 50.2). Results of TEG-PM were not significantly different for the 1-analyzer and 2-analyzer methods. High correlation was found between the 2 methods for MAfibrin [concordance correlation coefficient (r) = 0.930]; moderate correlation was found for MAthrombin (r = 0.70) and MAADP (r = 0.57); correlation between the 2 methods for MAAA was lower (r = 0.32). Thromboelastography platelet mapping (TEG-PM) should be further investigated to determine if it is a suitable method for measuring platelet dysfunction in dogs with thrombopathy.
本研究的目的是描述在20只健康犬中使用两种技术进行血栓弹力图血小板功能检测（TEG-PM）的结果。记录了凝血酶产生的最大振幅（MAthrombin）、纤维蛋白（MAfibrin）、二磷酸腺苷（ADP）受体活性（MAADP）和血栓素A2（TxA2）受体活性（由花生四烯酸刺激，MAAA）。血栓弹力图血小板功能检测按照制造商的指南（双分析仪技术）进行，并在从每只犬获取的两份独立血样上使用该方法的一种变体（单分析仪技术）。单分析仪/双分析仪技术的平均[±标准差（SD）]MA值为：MAthrombin = 51.9 mm（± 7.1）/52.5 mm（± 8.0）；MAfibrin = 20.7 mm（± 21.8）/23.0 mm（± 26.1）；MAADP = 44.5 mm（± 15.6）/45.6 mm（± 17.0）；MAAA = 45.7 mm（± 11.6）/45.0 mm（± 15.4）。由于ADP受体活性导致的平均（± SD）聚集百分比为70.4%（± 32.8）/67.6%（± 33.7）。由于TxA2受体活性导致的平均聚集百分比为77.3%（± 31.6）/78.1%（± 50.2）。单分析仪和双分析仪方法的TEG-PM结果无显著差异。两种方法在MAfibrin方面具有高度相关性[一致性相关系数（r）= 0.930]；在MAthrombin（r = 0.70）和MAADP（r = 0.57）方面具有中度相关性；两种方法在MAAA方面的相关性较低（r = 0.32）。应进一步研究血栓弹力图血小板功能检测（TEG-PM），以确定它是否是一种适用于测量患有血栓病犬的血小板功能障碍的方法。
