Jorbenadze Rezo, Schleicher Erwin, Bigalke Boris, Stellos Konstantinos, Gawaz Meinrad
Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard Karls-Universität , Tübingen , Germany and.
Platelets. 2014;25(6):409-15. doi: 10.3109/09537104.2013.829913. Epub 2013 Oct 8.
Platelet-bound stromal cell-derived factor-1 (SDF-1) plays a crucial role in attachment of circulating CD34(+) progenitor cells to the vascular wall, facilitating tissue healing after injury. However there is no evidence about expression of platelet-bound SDF-1 in patients with congestive heart failure (CHF). The aim of our study was to evaluate expression of platelet-bound SDF-1 and number of CD34(+) progenitor cells in patients with CHF. Forty-eight patients with idiopathic dilated cardiomyopathy (DCM) and 61 patients with ischaemic cardiomyopathy (ICM) were consecutively enrolled into the study. Blood taken from 109 consecutive patients was studied for surface expression of platelet-bound SDF-1 and number of CD34(+) progenitor cells by flow cytometry. The highest expression of platelet-bound SDF-1 was observed in patients with severe impairment of left ventricular systolic function compared with patients with mild or moderate impairment of left ventricular systolic function (mild vs. moderate vs. severe impairment of left ventricular systolic function: MFI ± SD: 35.6 ± 34 vs. 101.45 ± 73 vs. 124.86 ± 86.7, Kruskal-Wallis p < 0.001). Similar to platelet-bound SDF-1 number of CD34(+) progenitor cells was the highest in severe impairment of left ventricular systolic function (mild vs. moderate vs. severe impairment of left ventricular systolic function: mean ± SD: 260.4 ± 177.5 vs. 580.7 ± 340.5 vs. 640.82 ± 370.6, Kruskal-Wallis p < 0.001). Platelet-bound SDF-1 expression was associated with number of circulating CD34(+) progenitor cells (r = 0.454, p < 0.001) in patients with CHF. Expression of platelet-bound SDF-1 and number of CD34(+) cells were higher in patients with DCM compared with patients with ICM (p < 0.001 for both) and inversely correlated with age and aspirin therapy. Platelet-bound SDF-1 and CD34(+) progenitor cells are especially increased in patients with severe impairment of left ventricular systolic function in CHF.
血小板结合的基质细胞衍生因子-1（SDF-1）在循环中的CD34(+)祖细胞黏附于血管壁的过程中发挥关键作用，有助于损伤后的组织愈合。然而，尚无关于充血性心力衰竭（CHF）患者血小板结合SDF-1表达的证据。我们研究的目的是评估CHF患者血小板结合SDF-1的表达及CD34(+)祖细胞的数量。48例特发性扩张型心肌病（DCM）患者和61例缺血性心肌病（ICM）患者连续纳入本研究。通过流式细胞术对连续109例患者采集的血液进行血小板结合SDF-1的表面表达及CD34(+)祖细胞数量的研究。与左心室收缩功能轻度或中度受损的患者相比，左心室收缩功能严重受损的患者中血小板结合SDF-1的表达最高（左心室收缩功能轻度受损vs.中度受损vs.严重受损：平均荧光强度±标准差：35.6±34 vs. 101.45±73 vs. 124.86±86.7，Kruskal-Wallis检验p<0.001）。与血小板结合SDF-1相似，CD34(+)祖细胞数量在左心室收缩功能严重受损时最高（左心室收缩功能轻度受损vs.中度受损vs.严重受损：均值±标准差：260.4±177.5 vs. 580.7±340.5 vs. 640.82±370.6，Kruskal-Wallis检验p<0.001）。CHF患者中，血小板结合SDF-1的表达与循环CD34(+)祖细胞数量相关（r=0.454，p<0.001）。与ICM患者相比，DCM患者中血小板结合SDF-1的表达及CD34(+)细胞数量更高（两者p均<0.001），且与年龄和阿司匹林治疗呈负相关。CHF患者中，左心室收缩功能严重受损时血小板结合SDF-1及CD34(+)祖细胞尤其增加。
