Pratt J H, Turner D A, McAteer J A, Henry D P
Endocrinology. 1985 Sep;117(3):1189-94. doi: 10.1210/endo-117-3-1189.
Adrenal glomerulosa was examined for the presence of an adrenergic influence on aldosterone production. Cultured rat adrenal capsular explants were transferred to a perifusion system where the effect of exposure to catecholamines on aldosterone production was assessed. At 10(-6) M, isoproterenol greater than epinephrine greater than norepinephrine significantly stimulated aldosterone production, whereas at 10(-8) M only isoproterenol showed significant stimulation. Propranolol, a beta-adrenoreceptor antagonist, inhibited stimulation by epinephrine, and the phosphodiesterase inhibitor, 1-methyl-3-isobutylxanthine, enhanced stimulation by a submaximal dose of epinephrine. Epinephrine and norepinephrine were found by radioenzymatic assay to be present in fresh as well as cultured capsular tissue, although levels were considerably lower in tissue that had been in culture (about one tenth that of fresh tissue). The epinephrine-norepinephrine ratio was similar in capsule and medulla, suggesting a medullary source of capsular catecholamines. Whether catecholamines in the capsule arose from the in vitro manipulation of adrenal tissue or existed in vivo is unclear. In summary, beta-agonists stimulate aldosterone production in cultured rat capsular explants.
研究了肾上腺球状带是否存在对醛固酮生成的肾上腺素能影响。将培养的大鼠肾上腺被膜外植体转移至灌流系统，在该系统中评估暴露于儿茶酚胺对醛固酮生成的影响。在10⁻⁶ M时，异丙肾上腺素＞肾上腺素＞去甲肾上腺素显著刺激醛固酮生成，而在10⁻⁸ M时只有异丙肾上腺素显示出显著刺激作用。β-肾上腺素能受体拮抗剂普萘洛尔抑制肾上腺素的刺激作用，磷酸二酯酶抑制剂1-甲基-3-异丁基黄嘌呤增强亚最大剂量肾上腺素的刺激作用。通过放射酶法测定发现新鲜及培养的被膜组织中均存在肾上腺素和去甲肾上腺素，尽管培养组织中的水平显著低于新鲜组织（约为新鲜组织的十分之一）。被膜和髓质中的肾上腺素-去甲肾上腺素比值相似，提示被膜儿茶酚胺来源于髓质。尚不清楚被膜中的儿茶酚胺是源于肾上腺组织的体外操作还是体内就已存在。总之，β-激动剂刺激培养的大鼠被膜外植体中的醛固酮生成。
