Sequeira S J, McKenna T J
Endocrinology. 1985 Nov;117(5):1947-52. doi: 10.1210/endo-117-5-1947.
This study was undertaken to explore the possibility that the neurogenic amines epinephrine and norepinephrine may influence aldosterone production in vitro and to examine again the previously reported inhibitory effect of dopamine on aldosterone production. This was accomplished using bovine glomerulosa cell suspensions and a highly specific RIA for aldosterone. Epinephrine and norepinephrine (10(-6) - 10(-10) M) had no significant effect on aldosterone production. Both basal and angiotensin II-stimulated aldosterone production were significantly inhibited by dopamine, 10(-4) M, P less than 0.05. Basal aldosterone production was unaffected by lower concentrations of dopamine whereas angiotensin II-stimulated aldosterone production was inhibited in a dose-dependent manner that was significant to 10(-6) M dopamine (P less than 0.05). Pretreatment of glomerulosa cells with the dopamine antagonist metoclopramide impaired the inhibitory effect of dopamine on aldosterone production. This study supports the hypothesis that dopamine may be a significant inhibitor of aldosterone production in vivo. The other neurogenic amines studied, epinephrine and norepinephrine, had no significant effect on aldosterone production in vitro.
本研究旨在探讨神经源性胺类物质肾上腺素和去甲肾上腺素在体外是否可能影响醛固酮的产生，并再次检验先前报道的多巴胺对醛固酮产生的抑制作用。这是通过使用牛肾小球细胞悬液和一种高度特异性的醛固酮放射免疫分析法来完成的。肾上腺素和去甲肾上腺素（10^(-6) - 10^(-10) M）对醛固酮的产生没有显著影响。多巴胺（10^(-4) M）显著抑制基础状态和血管紧张素II刺激下的醛固酮产生，P < 0.05。较低浓度的多巴胺对基础醛固酮产生没有影响，而血管紧张素II刺激下的醛固酮产生则以剂量依赖方式受到抑制，至10^(-6) M多巴胺时仍具有显著性（P < 0.05）。用多巴胺拮抗剂甲氧氯普胺对肾小球细胞进行预处理会削弱多巴胺对醛固酮产生的抑制作用。本研究支持多巴胺可能是体内醛固酮产生的重要抑制剂这一假说。所研究的其他神经源性胺类物质，即肾上腺素和去甲肾上腺素，在体外对醛固酮的产生没有显著影响。
