Laboratory of Molecular Oncology Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad, India.
Eur J Clin Invest. 2013 Dec;43(12):1233-9. doi: 10.1111/eci.12163. Epub 2013 Sep 17.
The two main oesophageal cancer subtypes namely adenocarcinoma and squamous cell carcinoma exhibit interesting clinical, pathological and geographical variations with the former being more common in the West and the latter in Asia.
We evaluated status of p53, EGFR, Wnt and HPV in addition to microsatellite instability and loss of heterozygosity of several chromosomal loci in the two oesophageal cancer subtypes from India. The comparative analysis was extended to two oesophageal adenosquamous mixed cancer samples.
Our results reveal a high frequency of EGFR overexpression in ESCC as against EAC, while Wnt activation was a significantly more common event in EAC as against ESCC. Frequencies of p53 perturbations were not significantly different in the two subtypes. Interestingly, the EGFR and Wnt status in adenocarcinoma and squamous components of the two oesophageal adenosquamous cancer samples were identical to primary tumours. In addition, no common molecular aberration (including instability and loss of heterozygosity) in several microsatellites was detected in DNA isolated from the two components in both adenosquamous cancer samples.
Our results reveal the presence of distinct aberrations in oesophageal adenocarcinoma and squamous cell carcinoma which are replicated in the respective components of adenosquamous cancers. The study therefore suggests perhaps an independent origin of the two components of oesophageal adenosquamous mixed cancer.
两种主要的食管癌亚型，即腺癌和鳞状细胞癌，表现出有趣的临床、病理和地理变化，前者在西方更为常见，后者在亚洲更为常见。
我们评估了来自印度的两种食管癌亚型（即食管腺癌和食管鳞状细胞癌）中 p53、EGFR、Wnt 和 HPV 的状态，以及几个染色体位点的微卫星不稳定性和杂合性丢失。比较分析还扩展到了两种食管腺鳞混合癌样本。
我们的结果显示，在 ESCC 中 EGFR 的过度表达频率高于 EAC，而在 EAC 中 Wnt 的激活是一个更为常见的事件。两种亚型中 p53 改变的频率没有显著差异。有趣的是，两种食管腺鳞癌样本中腺癌和鳞状成分的 EGFR 和 Wnt 状态与原发性肿瘤相同。此外，在两个腺鳞癌样本的两个成分中分离的 DNA 中，没有检测到几个微卫星中常见的分子异常（包括不稳定性和杂合性丢失）。
我们的结果揭示了食管腺癌和鳞状细胞癌中存在明显的异常，这些异常在腺鳞癌的相应成分中得到了复制。因此，该研究表明食管腺鳞混合癌的两个成分可能具有独立的起源。
