Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Plant Biotechnol J. 2014 Jan;12(1):93-104. doi: 10.1111/pbi.12120. Epub 2013 Sep 16.
High temperature stress disturbs cellular homoeostasis and results in a severe retardation in crop growth and development. Thus, it is important to reveal the mechanism of plants coping with heat stress. In this study, a novel gene that we identified from Brassica napus, referred to as BnTR1, was found to play a key role in heat stress response in planta. BnTR1 is a membrane-bound RINGv (C₄HC₃) protein that displays E3 ligase activity in vitro. We demonstrated that modest expression of BnTR1 is sufficient to minimize adverse environmental influence and confers thermal resistance on development without any detrimental effects in B. napus and Oryza sativa. Our investigation into the action mechanism indicates that BnTR1 is likely to be involved in mediating Ca²⁺ dynamics by regulating the activity of calcium channels, which further alters the transcripts of heat shock factors and heat shock proteins contributing to plant thermotolerance. Hence, our study identified BnTR1 as a novel key factor underlying a conserved mechanism conferring thermal resistance in plants.
高温胁迫扰乱细胞内稳态，严重抑制作物生长和发育。因此，揭示植物应对热胁迫的机制非常重要。本研究从甘蓝型油菜中鉴定到一个新的基因 BnTR1，该基因在植物体内参与热胁迫应答。BnTR1 是一种膜结合的 RINGv（C₄HC₃）蛋白，在体外具有 E3 连接酶活性。我们证实适度表达 BnTR1 足以最小化环境胁迫的不利影响，并赋予油菜和水稻耐热性而无任何有害影响。我们对作用机制的研究表明，BnTR1 可能通过调节钙通道的活性来参与钙动态的调节，从而进一步改变热休克因子和热休克蛋白的转录本，有助于植物的耐热性。因此，本研究鉴定出 BnTR1 是赋予植物耐热性的保守机制中的一个新的关键因子。
