Yu Yang, Shao Bingyi, Shuai Yi, Shang Fengqing, Wang Xi, Zhou Zhifei, Liao Li, Jin Yan, Yang Deqin
Department of Endodontics, Affliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Oct;29(10):1028-31.
To investigate the difference in the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) of mice from ovariectomy (OVX) group and sham group in treating colitis, and then further study the differences of Fas/FasL expression and downstream T cell migration and apoptosis between the two groups.
The osteoporosis animal models were set up by ovariectomy in C57BL6 mice. Meanwhile, 3% dextran sulfate sodium (DSS) was administered for inducing colitis. We compared the therapeutic effects of BMSCs from OVX and sham groups in treating colitis, in addition, detected the expression of Fas/FasL in BMSCsby means of RT-PCR and Western blotting. The ability of BMSCs from the two groups of inducing T cell migaration and apoptosis was also detected.
Compared with the sham group, BMSCs from OVX mice expressed a lower level of Fas/FasL and displayed a decreased ability of inducing T cell migration and apoptosis, thus leading to an inferior therapeutic effect in treating colitis in animal models.
Fas/FasL expression of BMSCs from the OVX mice is down regulated, thus leading to a decrease of the migration and apoptosis for T cells from mouse colitis.
探讨去卵巢（OVX）组和假手术组小鼠骨髓间充质干细胞（BMSCs）治疗结肠炎的疗效差异，并进一步研究两组Fas/FasL表达及下游T细胞迁移和凋亡的差异。
通过对C57BL6小鼠进行去卵巢手术建立骨质疏松动物模型。同时，给予3%硫酸葡聚糖钠（DSS）诱导结肠炎。比较OVX组和假手术组BMSCs治疗结肠炎的疗效，此外，通过RT-PCR和蛋白质印迹法检测BMSCs中Fas/FasL的表达。还检测了两组BMSCs诱导T细胞迁移和凋亡的能力。
与假手术组相比，OVX小鼠的BMSCs Fas/FasL表达水平较低，诱导T细胞迁移和凋亡的能力降低，从而导致在动物模型中治疗结肠炎的疗效较差。
OVX小鼠BMSCs的Fas/FasL表达下调，从而导致小鼠结肠炎T细胞的迁移和凋亡减少。
