采用酶联免疫吸附测定（ELISA）法测定，接受香豆素治疗的患者中，凝血因子X抗原水平高于其凝血活性水平。

Excess of factor X antigen versus clotting counterpart in coumarin treated patients as determined by an Elisa method.

作者信息

Girolami A, Vicarioto M A, Ruzza G, Vergolani A, Boeri G

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1985;112(2):326-32.

Abstract

Factor X antigen was assayed by means of an Elisa (sandwich) method in 36 patients on long-term anticoagulant therapy. The average value observed was 31.4% +/- 12.2. In almost every instance the antigen level was higher than the clotting counterpart (14.4% +/- 4.5). In a few instances no major difference was noted between factor X antigen and factor X activity. The method correlated fairly well with other immunological methods (electroimmunoassay and Laser Nephelometer). Therefore, factor X Elisa method appears to be a suitable method for factor X antigen evaluation.

摘要

采用酶联免疫吸附测定（夹心）法对36例接受长期抗凝治疗的患者进行了X因子抗原检测。观察到的平均值为31.4%±12.2。几乎在每一个病例中，抗原水平都高于凝血对应值（14.4%±4.5）。在少数病例中，未发现X因子抗原与X因子活性之间有显著差异。该方法与其他免疫方法（电免疫测定法和激光散射比浊法）相关性良好。因此，X因子酶联免疫吸附测定法似乎是评估X因子抗原的合适方法。