Santi P A, Anderson C B
Hear Res. 1987;27(1):47-65. doi: 10.1016/0378-5955(87)90025-6.
Routine electron microscope methods do not well preserve or stain the surface coat or glycocalyx on cochlear hair cells. In other tissues, enhanced preservation and staining of these glycoconjugates was obtained following fixation with glutaraldehyde containing a cationic dye (e.g., Alcian blue and ruthenium red). When cochleas were fixed with glutaraldehyde containing Alcian blue, the endolymphatic surface of hair cells, but not the supporting cells, displayed an extensive (approximately 90 nm thick) surface coat. Alcian blue positive material was also observed in the tectorial and basilar membranes and in a portion of the spiral ligament. In addition, acellular bands of Alcian blue positive material were observed between the tectorial membrane and the reticular lamina or inner sulcus cells. Although the function of these cochlear glycoconjugates is not yet known, it is proposed that they serve to attach the tectorial membrane to the organ of Corti, and they are involved in stereocilia fusion following sound exposure and ototoxic drug administration.
常规电子显微镜方法不能很好地保存或染色耳蜗毛细胞的表面被膜或糖萼。在其他组织中，在用含有阳离子染料（如阿尔辛蓝和钌红）的戊二醛固定后，这些糖缀合物的保存和染色得到了增强。当用含有阿尔辛蓝的戊二醛固定耳蜗时，毛细胞的内淋巴表面而非支持细胞显示出广泛的（约90纳米厚）表面被膜。在盖膜和基底膜以及部分螺旋韧带中也观察到阿尔辛蓝阳性物质。此外，在盖膜与网状板或内沟细胞之间观察到无细胞的阿尔辛蓝阳性物质带。虽然这些耳蜗糖缀合物的功能尚不清楚，但有人提出它们有助于将盖膜附着到柯蒂器上，并且它们参与声音暴露和耳毒性药物给药后的静纤毛融合。
