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2
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本文引用的文献

1
AN OPTICAL METHOD FOR THE ANALYSIS OF PERIODICITIES IN ELECTRON MICROGRAPHS, AND SOME OBSERVATIONS ON THE MECHANISM OF NEGATIVE STAINING.一种分析电子显微照片中周期性的光学方法，以及关于负染色机制的一些观察结果。
J Mol Biol. 1964 Dec;10:565-9. doi: 10.1016/s0022-2836(64)80081-4.
2
Structure of Limulus telson muscle thick filaments.鲎尾节肌粗肌丝的结构。
J Mol Biol. 1981 Dec 15;153(3):781-90. doi: 10.1016/0022-2836(81)90418-6.
3
A new model for the geometry of the binding of myosin crossbridges to muscle thin filaments.肌球蛋白横桥与肌肉细肌丝结合的几何结构新模型。
J Mol Biol. 1981 Apr 5;147(2):297-324. doi: 10.1016/0022-2836(81)90442-3.
4
An electron microscopic and optical diffraction analysis of the structure of Limulus telson muscle thick filaments.鲎尾节肌粗肌丝结构的电子显微镜和光学衍射分析。
J Cell Biol. 1982 Feb;92(2):443-51. doi: 10.1083/jcb.92.2.443.
5
Organization of myosin in invertebrate thick filaments.无脊椎动物粗肌丝中肌球蛋白的组织
Soc Gen Physiol Ser. 1982;37:29-36.
6
Structure of the thick filaments in molluscan adductor muscle.软体动物闭壳肌中粗肌丝的结构。
Soc Gen Physiol Ser. 1982;37:11-28.
7
A model of myosin crossbridge structure consistent with the low-angle x-ray diffraction pattern of vertebrate muscle.一种与脊椎动物肌肉的低角度X射线衍射图谱相符的肌球蛋白横桥结构模型。
J Muscle Res Cell Motil. 1980 Jun;1(2):177-91. doi: 10.1007/BF00711798.
8
Determination of the handedness of the crossbridge helix of Limulus thick filaments.鲎粗肌丝横桥螺旋手性的测定。
J Muscle Res Cell Motil. 1982 Sep;3(3):349-61. doi: 10.1007/BF00713042.
9
Structure of myosin/paramyosin filaments from a molluscan smooth muscle.来自软体动物平滑肌的肌球蛋白/副肌球蛋白丝的结构。
J Mol Biol. 1983 Jul 15;167(4):853-72. doi: 10.1016/s0022-2836(83)80115-6.
10
Electron microscopy and image analysis of myosin filaments from scallop striated muscle.扇贝横纹肌肌球蛋白丝的电子显微镜观察与图像分析
J Mol Biol. 1983 Apr 5;165(2):303-20. doi: 10.1016/s0022-2836(83)80259-9.

蝎肌粗肌丝结构的电子显微镜及光学衍射分析

Electron microscopic and optical diffraction analysis of the structure of scorpion muscle thick filaments.

作者信息

Kensler R W, Levine R J, Stewart M

出版信息

J Cell Biol. 1985 Aug;101(2):395-401. doi: 10.1083/jcb.101.2.395.

DOI:10.1083/jcb.101.2.395
PMID:2410429
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2113686/
Abstract

We rapidly and gently isolated thick filaments from scorpion tail muscle by a modification of the technique previously described for isolating Limulus thick filaments. Images of negatively stained filaments appeared to be highly periodic, with a well-preserved myosin cross-bridge array. Optical diffraction patterns of the electron micrograph images were detailed and similar to optical diffraction patterns from Limulus and tarantula thick filaments. Analysis of the optical diffraction patterns and computed Fourier transforms, together with the appearance of the filaments in the micrographs, suggested a model for the filaments in which the myosin cross-bridges were arranged on four helical strands with 12 cross-bridges per turn of each strand, thus giving the observed repeat every third cross-bridge level. Comparison of the scorpion thick filaments with those isolated from the closely related chelicerate arthropods, Limulus and tarantula, revealed that they were remarkably similar in appearance and helical symmetry but different in diameter.

摘要

我们通过改进先前用于分离鲎粗肌丝的技术，快速且轻柔地从蝎尾肌肉中分离出粗肌丝。负染肌丝的图像显示出高度周期性，肌球蛋白横桥阵列保存完好。电子显微镜图像的光学衍射图案详细且类似于鲎和狼蛛粗肌丝的光学衍射图案。对光学衍射图案和计算出的傅里叶变换的分析，以及显微照片中肌丝的外观，提出了一个肌丝模型，其中肌球蛋白横桥排列在四条螺旋链上，每条链每转有12个横桥，从而在每第三个横桥水平产生观察到的重复结构。将蝎粗肌丝与从密切相关的螯肢节肢动物鲎和狼蛛中分离出的粗肌丝进行比较，发现它们在外观和螺旋对称性上非常相似，但直径不同。