White T R, Lee C S, French P, Hewish D, Brandon M R
J Histochem Cytochem. 1985 Aug;33(8):785-90. doi: 10.1177/33.8.2410481.
A monoclonal antibody (SBU-1) was raised to sheep thymic rudiment by fusion of NSI myeloma cells with spleen cells from BALB/c mice immunized with thymic rudiment isolated from fetal sheep between 25-30 days of gestation. By employing the indirect immunoperoxidase technique the antigen recognized by SBU-1 was found to be present in the epithelial reticular cells of the fetal sheep thymus. The intensity of staining decreased as gestation progressed. In the adult thymus the antigen was mainly restricted to Hassall's corpuscles and occasional epithelial cells in the medulla. In addition, the antigen was also shown to be present in epithelial cells of the small intestine, the bronchiole, the keratinized epithelium of the rumen, and the epithelial cells of the kidney tubules. By use of immunofluorescence the antigen was shown to be present in most of the cells of wool follicles and the cortex of developing wool fibers. Western blotting of SBU-1 against the low-sulfur alpha-keratin proteins of wool confirmed that the antigen recognized by SBU-1 belongs to a family of keratins. It was concluded that SBU-1 was raised against alpha-keratin expressed by the epithelial cells of the thymic rudiment and that the expression of this antigen on the reticular network of the thymus declined with advancement of pregnancy.
通过将NSI骨髓瘤细胞与用妊娠25至30天的胎羊胸腺原基免疫的BALB/c小鼠的脾细胞融合，制备了一种针对绵羊胸腺原基的单克隆抗体（SBU-1）。采用间接免疫过氧化物酶技术发现，SBU-1识别的抗原存在于胎羊胸腺的上皮网状细胞中。随着妊娠进展，染色强度降低。在成年胸腺中，该抗原主要局限于哈氏小体和髓质中的偶尔上皮细胞。此外，该抗原还显示存在于小肠上皮细胞、细支气管、瘤胃角化上皮和肾小管上皮细胞中。通过免疫荧光法显示，该抗原存在于毛囊的大多数细胞和发育中的羊毛纤维的皮质中。用SBU-1对羊毛的低硫α-角蛋白进行蛋白质免疫印迹分析证实，SBU-1识别的抗原属于角蛋白家族。得出的结论是，SBU-1是针对胸腺原基上皮细胞表达的α-角蛋白产生的，并且随着妊娠进展，该抗原在胸腺网状结构上的表达下降。
