Key Laboratory of Plant Resources Conservation And Sustainable Utilization, South China Botanical Garden, Chinese Academy Of Sciences , Guangzhou 510650, People's Republic of China.
J Agric Food Chem. 2013 Oct 23;61(42):10091-5. doi: 10.1021/jf4030882. Epub 2013 Oct 9.
The antifungal activity of a natural resorcylic acid lactone, hypothemycin (HPM), against Peronophythora litchii in vitro and in vivo was investigated. HPM treatment substantially suppressed spore germination of P. litchi, with the inhibition rate of 100% when 0.78 μg/mL HPM was applied. Similarly, mycelial growth of P. litchii was efficiently inhibited. Furthermore, HPM caused the ultrastructural modifications of P. litchii, including the disruption of the cell wall and the endomembrane system, especially the plasma membrane, mitochondria, and vacuoles, which led to the destruction of the cellular integrity. Moreover, application of HPM significantly reduced decay and suppressed peel browning of postharvest litchi fruit inoculated with P. litchii during storage at 28 °C. Overall, these findings suggested that HPM exhibited excellent antifungal activity against P. litchii both in vitro and in vivo, which could be helpful for the storage of harvest litchi fruit.
研究了天然雷琐酚内酯 Hypothemycin（HPM）对李拟茎点霉的体外和体内抗真菌活性。HPM 处理可显著抑制李拟茎点霉孢子的萌发，当应用 0.78μg/mL HPM 时，抑制率达到 100%。同样，李拟茎点霉的菌丝生长也得到有效抑制。此外，HPM 导致李拟茎点霉的超微结构发生改变，包括细胞壁和内膜系统的破坏，特别是质膜、线粒体和液泡，导致细胞完整性被破坏。此外，HPM 的应用显著减少了腐烂，并抑制了在 28°C 下贮藏期间接种李拟茎点霉的荔枝果实的果皮褐变。总的来说，这些发现表明 HPM 对李拟茎点霉具有良好的体外和体内抗真菌活性，这可能有助于收获荔枝果实的贮藏。
