Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham, 44150, Thailand.
Mol Ecol Resour. 2014 Mar;14(2):262-71. doi: 10.1111/1755-0998.12174. Epub 2013 Oct 21.
The ecological and medical importance of black flies drives the need for rapid and reliable identification of these minute, structurally uniform insects. We assessed the efficiency of DNA barcoding for species identification of tropical black flies. A total of 351 cytochrome c oxidase subunit 1 sequences were obtained from 41 species in six subgenera of the genus Simulium in Thailand. Despite high intraspecific genetic divergence (mean = 2.00%, maximum = 9.27%), DNA barcodes provided 96% correct identification. Barcodes also differentiated cytoforms of selected species complexes, albeit with varying levels of success. Perfect differentiation was achieved for two cytoforms of Simulium feuerborni, and 91% correct identification was obtained for the Simulium angulistylum complex. Low success (33%), however, was obtained for the Simulium siamense complex. The differential efficiency of DNA barcodes to discriminate cytoforms was attributed to different levels of genetic structure and demographic histories of the taxa. DNA barcode trees were largely congruent with phylogenies based on previous molecular, chromosomal and morphological analyses, but revealed inconsistencies that will require further evaluation.
黑蝇具有重要的生态和医学意义,因此需要快速、可靠地识别这些微小、结构一致的昆虫。我们评估了 DNA 条形码技术在热带黑蝇种鉴定中的效率。从泰国六个拟蚊属亚属的 41 个种中共获得了 351 个细胞色素 c 氧化酶亚基 1 序列。尽管种内遗传分化很高(平均值为 2.00%,最大值为 9.27%),但 DNA 条形码提供了 96%的正确鉴定。条形码还区分了选定的种复合体的细胞型,尽管成功率不同。两种 Feuerborni 拟蚊的细胞型可以实现完美区分,而对 angulistylum 复合体的识别成功率为 91%。然而,对于 siamense 复合体的识别成功率较低(33%)。DNA 条形码区分细胞型的效率不同,这归因于分类单元的遗传结构和种群历史水平不同。DNA 条形码树与基于先前的分子、染色体和形态学分析的系统发育基本一致,但也存在一些需要进一步评估的不一致之处。