Zhao Ya-Peng, Pan Xiao-Fu, Yang Jun-Xing, Chen Xiao-Yong, Wang Xiao-Ai, Li Zai-Yun
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China.
Dongwuxue Yanjiu. 2013 Oct;34(5):499-503.
In the present study, we examined the potential uses of Alizarin complexone and Alizarin red S to mark S. grahami larvae and juveniles. Individuals of different ages (6 days, 20 days, 90 days) were immersed in four concentrations of a solution of each chemical (50, 100, 150 and 200 mg/L) for different periods of time (4-36 h). Marked individuals were euthanized 4-27 days after immersion, and the lapillus was removed and used to determine mark quality. After marking with 50-150 mg/L ALC and ARS, violet marks were visible under normal light, and scarlet fluorescent marks were visible under 510-560 nm green light. The clear marks under normal light were recorded, and a 100% survival rate was guaranteed under the following conditions: 6 day old larvae immersed in 50 mg/L ALC or ARS for 8h; 20 day old larvae immersed in 50 mg/L ALC or 100 mg/L ARS for 24h; 90 day old juveniles immersed in 100mg/L ALC or 150 mg/L ARS for 24 h.
在本研究中，我们检测了茜素氨羧络合剂和茜素红S标记稀有鮈鲫仔鱼和幼鱼的潜在用途。将不同年龄（6天、20天、90天）的个体浸入每种化学物质的四种浓度溶液（50、100、150和200 mg/L）中不同时间（4 - 36小时）。标记后的个体在浸入后4 - 27天进行安乐死处理，然后取出内耳石用于确定标记质量。用50 - 150 mg/L的茜素氨羧络合剂（ALC）和茜素红S（ARS）标记后，在正常光线下可见紫色标记，在510 - 560 nm绿光下可见猩红色荧光标记。记录正常光线下清晰的标记，在以下条件下保证100%的存活率：6日龄仔鱼浸入50 mg/L的ALC或ARS中8小时；20日龄仔鱼浸入50 mg/L的ALC或ARS中100 mg/L中24小时；90日龄幼鱼浸入100 mg/L的ALC或150 mg/L的ARS中24小时。
