Department of Cardiology and Coronary Unit, University Clinical Hospital of Santiago de Compostela, Santiago de Compostela, Spain.
Eur J Clin Invest. 2014 Jan;44(1):29-37. doi: 10.1111/eci.12186. Epub 2013 Nov 14.
Heart failure (HF) is associated with a pro-inflammatory state in epicardial fat, but the involved mechanisms are not entirely clear. The aim of our study was to assess the relationship between p53 and adiponectin mRNA in epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT) in patients with heart failure and its sympathetic regulation.
Epicardial adipose tissue and SAT samples were obtained from 63 patients undergoing elective cardiac surgery. EAT and SAT explants culture from seven patients were stimulated with isoprenaline 0.1 or 1 uM for 6 h. p53 and adiponectin mRNA expression was measured in frozen biopsies or explants culture from both fat pads by real-time polymerase chain reaction (PCR).
We observed that EAT expressed more p53 mRNA than SAT (1.73 ± 0.07 vs. 1.69 ± 0.04, P < 0.001) and its levels were higher in HF patients (1.75 ± 0.07 vs. 1.70 ± 0.04, P < 0.01 in EAT and 1.70 ± 0.04 vs. 1.67 ± 0.04, P < 0.05 in SAT). Moreover, p53 mRNA expression was negatively correlated with adiponectin in EAT. After analysing the p53 mRNA regulation by isoprenaline, we observed that only EAT p53 expression increased after adrenergic stimulation (1.63 ± 0.01 vs. 1.66 ± 0.02; P = 0.024).
p53 mRNA expression levels, inversely correlated with adiponectin, increase in EAT of HF patients and can be regulated by sympathetic activation pathway. Our findings can help to explain the deleterious effect of sympathetic activation in HF.
心力衰竭（HF）与心外膜脂肪的促炎状态有关，但涉及的机制尚不完全清楚。我们的研究目的是评估心力衰竭患者心外膜脂肪组织（EAT）和皮下脂肪组织（SAT）中 p53 和脂联素 mRNA 之间的关系及其交感神经调节。
从 63 例行择期心脏手术的患者中获取心外膜脂肪组织和 SAT 样本。对 7 例患者的 EAT 和 SAT 标本进行异丙肾上腺素 0.1 或 1 uM 刺激 6 小时。通过实时聚合酶链反应（PCR）测量冷冻活检或来自两个脂肪垫的培养物中的 p53 和脂联素 mRNA 表达。
我们观察到 EAT 表达的 p53 mRNA 多于 SAT（1.73±0.07 比 1.69±0.04，P<0.001），HF 患者的水平更高（EAT 中 1.75±0.07 比 1.70±0.04，P<0.01；SAT 中 1.70±0.04 比 1.67±0.04，P<0.05）。此外，EAT 中的 p53 mRNA 表达与脂联素呈负相关。分析异丙肾上腺素对 p53 mRNA 的调节后，我们观察到仅 EAT 的 p53 表达在肾上腺素能刺激后增加（1.63±0.01 比 1.66±0.02；P=0.024）。
HF 患者 EAT 中 p53 mRNA 表达水平与脂联素呈负相关，且可通过交感神经激活途径调节。我们的发现可以帮助解释交感神经激活对 HF 的有害影响。
