Taylor C E, Baker P J
J Immunol. 1985 Oct;135(4):2551-6.
Supernatant fluid (SF) derived from spleen cell cultures, obtained from mice 16 hr after immunization with 0.5 microgram of Type III pneumococcal polysaccharide (SSS-III), suppressed the antibody response when SF was given (i.v.) 3 hr before immunization with SSS-III. Such suppression was antigen specific and could be reproduced by SF derived from cultures of T cells from mice immunized with SSS-III (0.5 microgram) or by SF derived from cultures of spleen cells from mice primed with a subimmunogenic dose of SSS-III (0.005 microgram). Adsorption of SF with SSS-III covalently bound to a Sepharose 4B column did not alter the ability of SF to suppress the SSS-III-specific antibody response. However, adsorption of SF with Ig+ (B) cells from mice immunized with 0.5 microgram SSS-III completely removed the suppressive activity. Significant (p less than 0.05) suppression of the antibody response was observed only when SF was administered (i.v.) 24 hr before to 24 hr after immunization with 0.5 microgram of SSS-III. These results suggest that suppressor T cells generated in response to SSS-III function by releasing a soluble factor(s) that binds to determinants on B cells rather than antigen; this soluble factor(s) acts directly on antigen-stimulated B cells or inhibits the induction of amplifier T cells.
用0.5微克Ⅲ型肺炎球菌多糖（SSS-Ⅲ）免疫小鼠16小时后，从脾细胞培养物中获得的上清液（SF），在以SSS-Ⅲ免疫前3小时静脉注射时，可抑制抗体反应。这种抑制具有抗原特异性，并且可用来自以SSS-Ⅲ（0.5微克）免疫的小鼠的T细胞培养物的SF，或用来自以亚免疫原剂量的SSS-Ⅲ（0.005微克）致敏的小鼠的脾细胞培养物的SF来重现。用共价结合到琼脂糖4B柱上的SSS-Ⅲ吸附SF，不会改变SF抑制SSS-Ⅲ特异性抗体反应的能力。然而，用来自以0.5微克SSS-Ⅲ免疫的小鼠的Ig⁺（B）细胞吸附SF，可完全消除其抑制活性。仅当在以0.5微克SSS-Ⅲ免疫前24小时至免疫后24小时静脉注射SF时，才观察到抗体反应受到显著（p<0.05）抑制。这些结果表明，对SSS-Ⅲ产生反应而生成的抑制性T细胞，通过释放一种可与B细胞上的决定簇而非抗原结合的可溶性因子发挥作用；这种可溶性因子直接作用于抗原刺激的B细胞或抑制放大性T细胞的诱导。
