Miller S D
J Immunol. 1984 Dec;133(6):3112-20.
This report defines a methodology for the production and characterization of an antigen-specific, monoclonal T cell hybrid-derived suppressor T cell factor (TsF) that suppresses the passive transfer of 2,4-dinitrofluorobenzene (DNFB) contact hypersensitivity. Fusion of T cells from BALB/c (H-2d) mice tolerized with syngeneic DNP-spleen cells to BW 5147 thymoma cells resulted in several hybrids that constitutively produce a soluble regulatory molecule. One of these hybrids, 26.10.2, was subsequently cloned, and its soluble factor was characterized with respect to its antigen specificity, biochemical nature, MHC restriction pattern, and identity of its target cell. 26.10.2 TsF suppresses the passive transfer of delayed-type hypersensitivity (DTH) mediated by DNP- but not trinitrochlorobenzene- or oxazalone-primed DTH T cells (TDH) after a 1 hr incubation at 37 degrees C. In contrast, 26.10.2 TsF had no suppressive effect on secondary in vitro DNP-specific T cell proliferative responses. 26.10.2 TsF therefore represents an antigen-specific factor with effector (efferent-acting) function. The monoclonal TsF was shown to consist of a two-chain, disulfide-bonded molecule, and to bear a receptor(s) specific for DNP and determinants encoded by the I region of the H-2 complex. Effector suppressive activity of 26.10.2 TsF was restricted by Class I H-2Dd determinants. One cellular target of this monoclonal factor was shown to be the DNP-specific TDH cell, because DNFB-primed lymph node cells from cyclophosphamide-pretreated donors (lacking Ts-auxiliary (Ts-aux) cells) were efficiently suppressed. The TsF appears to focus on passively bound, TDH receptor-associated, DNP-Class I determinants, as suggested by the observation that freshly prepared, but not overnight cultured, DNP-specific TDH cells were susceptible to suppression.
本报告定义了一种生产和鉴定抗原特异性、单克隆T细胞杂交来源的抑制性T细胞因子（TsF）的方法，该因子可抑制2,4-二硝基氟苯（DNFB）接触性超敏反应的被动转移。用同基因DNP-脾细胞耐受的BALB/c（H-2d）小鼠的T细胞与BW 5147胸腺瘤细胞融合，产生了几种组成性产生可溶性调节分子的杂交细胞。其中一种杂交细胞26.10.2随后被克隆，并对其可溶性因子的抗原特异性、生化性质、MHC限制模式及其靶细胞的特性进行了鉴定。26.10.2 TsF在37℃孵育1小时后，可抑制由DNP致敏而非三硝基氯苯或恶唑酮致敏的迟发型超敏反应（DTH）T细胞（TDH）介导的迟发型超敏反应的被动转移。相比之下，26.10.2 TsF对体外二次DNP特异性T细胞增殖反应没有抑制作用。因此，26.10.2 TsF代表一种具有效应（传出作用）功能的抗原特异性因子。该单克隆TsF显示为由两条链通过二硫键连接的分子，并带有对DNP和由H-2复合体I区编码的决定簇具有特异性的受体。26.10.2 TsF的效应抑制活性受I类H-2Dd决定簇的限制。该单克隆因子的一个细胞靶标被证明是DNP特异性TDH细胞，因为来自环磷酰胺预处理供体（缺乏Ts辅助（Ts-aux）细胞）的DNFB致敏淋巴结细胞被有效抑制。正如观察到新鲜制备而非过夜培养的DNP特异性TDH细胞易受抑制所表明的那样，TsF似乎聚焦于被动结合的、与TDH受体相关的DNP-I类决定簇。
