Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Lodzki 1, 10-727, Olsztyn, Poland.
Mol Ecol Resour. 2014 Mar;14(2):272-85. doi: 10.1111/1755-0998.12175. Epub 2013 Oct 25.
The aim of this work was to evaluate the suitability of selected DNA regions in the barcoding of plants, based on the species belonging to the genus Lamium (Lamiaceae). For this purpose, nine chloroplast barcodes, that is, accD, matK, rbcL, rpoA, rpoB, rpoC1, rpoC2, trnH-psbA, trnL-trnF, as well as ITS nuclear region, and intron of mitochondrial nad5 gene were tested. Among the single-locus barcodes, most effective in the identification of Lamium species was the trnH-psbA spacer and matK gene. The high level of variability and resolving power was also observed in the case of rpoA and rpoC2 genes. Despite the high interspecies variability of ITS region, it turned out to be inapplicable in Lamium identification. An important disadvantage of ITS as a barcode is a limitation of its use in polyploid plants, samples contaminated with fungal material or samples with partially degraded DNA. We have also evaluated five-two-locus and two-three-locus barcode regions created from a combination of most effective single loci. The best-performing barcode combinations were matK + trnH-psbA and matK + rpoA. Both of them had equally high discriminative power to identify Lamium species.
本研究旨在评估基于 Lamium 属（Lamiaceae）物种的九个叶绿体条形码，即 accD、matK、rbcL、rpoA、rpoB、rpoC1、rpoC2、trnH-psbA、trnL-trnF 以及核 ITS 区和线粒体 nad5 基因内含子，在植物条形码中的适用性。在单基因座条形码中，鉴定 Lamium 种最有效的是 trnH-psbA 间隔区和 matK 基因。rpoA 和 rpoC2 基因也表现出较高的变异性和分辨率。尽管 ITS 区的种间变异性很高，但它不适用于 Lamium 的鉴定。ITS 作为条形码的一个重要缺点是，它在多倍体植物、真菌污染的样本或部分降解 DNA 的样本中无法使用。我们还评估了由最有效单基因座组合而成的五个双基因座和两个三基因座条形码区。表现最好的条形码组合是 matK+trnH-psbA 和 matK+rpoA。它们都具有同样高的鉴别力来识别 Lamium 种。
