Fahmy J A, Moller S, Bentzon M W
Acta Ophthalmol (Copenh). 1975 Jun;53(3):476-94. doi: 10.1111/j.1755-3768.1975.tb01178.x.
The peroperative flora of 499 patients undergoing cataract extraction was studied with local bacterial cultures taken at the beginning and end of surgery and compared with the preoperative flora examined previously (Fahmy et al. 1975 b) on admission the day prior to surgery. The local application of a single dose of oxytetracycline - polymyxin B, approximately 18 hours before surgery, significantly reduced the incidence of bacteria at the time of surgery. However, 92% of the conjunctivas examined immediately before operation proved to harbour one or more kinds of microorganisms. Futhermore, 61% of the wound sites were found to be contaminated with bacteria at the conclusion of surgery. The reasons are discussed. The origin of Staphylococcus aureus isolated peroperatively from the conjunctiva and wound site was studied. The great majority of strains could be traced to the patient's own conjunctiva preoperatively. In a few cases S. aureus was traced to the patient's own nose, skin of face or to the surgeon's nose. The air of the wards and operating theatre as well as the hands and gloves of surgeons and assistant nurses apparently did not play any role as a source of S. aureus infection.
对499例接受白内障摘除术患者的术中菌群进行了研究，在手术开始和结束时采集局部细菌培养样本，并与术前（手术前一天入院时）检测的术前菌群（Fahmy等人，1975年b）进行比较。在手术前约18小时局部应用单剂量土霉素 - 多粘菌素B，显著降低了手术时的细菌感染率。然而，在手术即将开始前检查的结膜中，92%被证明含有一种或多种微生物。此外，在手术结束时发现61%的伤口部位被细菌污染。文中对原因进行了讨论。对术中从结膜和伤口部位分离出的金黄色葡萄球菌的来源进行了研究。绝大多数菌株术前可追溯到患者自身的结膜。在少数情况下，金黄色葡萄球菌可追溯到患者自身的鼻子、面部皮肤或外科医生的鼻子。病房和手术室的空气以及外科医生和助理护士的手和手套显然不是金黄色葡萄球菌感染的来源。
