Ibrahim G F, Fleet G H, Lyons M J, Walker R A
Med Microbiol Immunol. 1985;174(2):101-13. doi: 10.1007/BF02123231.
Ten antisera raised in rabbits, against polymeric flagellins from ten different Salmonella serotypes were used to determine the relative cross-activities between salmonella flagellins in monomeric form. The results showed a high degree of cross-reactivity between the antisera (IgG antibodies) and all monomeric flagellins investigated. Consequently, it was possible to detect the ten Salmonella serotypes, after heat depolymerization of flagella using radioimmunometric assay with only one antiserum raised against polymeric flagellin from one serotype. The results also showed that native flagella differed antigenically from repolymerized and monomeric flagellins. This is possibly due to changes in the tertiary structure of sub-units of flagella when depolymerized with acid or heat resulting in unfolding and unmasking of common antigenic determinants. The unfolded and unmasked antigenic determinants were not only common to the Salmonella serotypes investigated, but also to other members of Enterobacteriaceae.
用十只兔子制备的针对十种不同沙门氏菌血清型的聚合鞭毛蛋白的抗血清，来测定单体形式的沙门氏菌鞭毛蛋白之间的相对交叉活性。结果显示抗血清（IgG抗体）与所有研究的单体鞭毛蛋白之间具有高度交叉反应性。因此，在使用针对一种血清型的聚合鞭毛蛋白产生的一种抗血清，通过放射免疫测定法对鞭毛进行热解聚后，就有可能检测出这十种沙门氏菌血清型。结果还表明，天然鞭毛在抗原性上与再聚合和单体鞭毛蛋白不同。这可能是由于用酸或热解聚时鞭毛亚基的三级结构发生变化，导致共同抗原决定簇展开并暴露。展开和暴露的抗原决定簇不仅在所研究的沙门氏菌血清型中常见，在肠杆菌科的其他成员中也常见。
