针对致病性大肠杆菌束状菌毛的单克隆抗体。
Monoclonal antibodies specific for the bundle-forming pilus of enteropathogenic Escherichia coli.
作者信息
Girón J A, Qadri F, Azim T, Jarvis K J, Kaper J B, Albert M J
机构信息
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore 21201, USA.
出版信息
Infect Immun. 1995 Dec;63(12):4949-52. doi: 10.1128/iai.63.12.4949-4952.1995.
Abstract
The bundle-forming pilus (BFP) produced by enteropathogenic Escherichia coli (EPEC) is associated with the presence of a large EPEC adherence factor plasmid and the formation of localized adherence clusters on tissue culture cells. Three mouse monoclonal antibodies (ICA2, ICA3, and ICA4) were produced against BFP purified from EPEC B171 (O111:NM). These monoclonal antibodies reacted in immunoblots with different epitopes of the 19.5-kDa bundlin subunit of BFP of heterologous EPEC. These reagents could serve as diagnostic tools for the identification of EPEC as well as for studying the role of BfpA in the interaction of EPEC with eukaryotic cells.
摘要
肠致病性大肠杆菌（EPEC）产生的束状菌毛（BFP）与一个大型EPEC黏附因子质粒的存在以及在组织培养细胞上形成局部黏附簇有关。针对从EPEC B171（O111:NM）纯化的BFP制备了三种小鼠单克隆抗体（ICA2、ICA3和ICA4）。这些单克隆抗体在免疫印迹中与异源EPEC的BFP的19.5-kDa菌毛束蛋白亚基的不同表位发生反应。这些试剂可作为鉴定EPEC的诊断工具，也可用于研究BfpA在EPEC与真核细胞相互作用中的作用。
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