Microsystems and BioMEMS Laboratory, University of Cincinnati, Cincinnati, OH 45221, USA.
Lab Chip. 2013 Dec 7;13(23):4653-62. doi: 10.1039/c3lc50403d.
A new sample-to-answer polymer lab-on-a-chip, which can perform immunoassay with minimum user intervention through on-chip reservoirs for reagents and single-channel assay system, has been designed, developed and successfully characterized as a point-of-care testing (POCT) cartridge for the detection of thyroid stimulating hormone (TSH). Test results were obtained within 30 minutes after a sample was dropped into the POCT cartridge. The analyzed results of TSH showed a linear range of up to 55 μIU mL(-1) with the limit of detection (LOD) of 1.9 μIU mL(-1) at the signal-to-noise ratio (SNR) of 3. The reagents stored in the on-chip reservoirs maintained more than 97% of their initial volume for 120 days of storage time while the detection antibody retained its activity above 98% for 120 days. The sample-to-answer polymer lab-on-a-chip developed in this work using the mass-producible and low-cost polymer is well suited for the point-of-care testing of rapid in vitro diagnostics (IVD) of TSH.
一种新的样本到答案聚合物芯片实验室，通过用于试剂的芯片上储液器和单通道分析系统，可以在最小的用户干预下进行免疫测定，已被设计、开发并成功地用作即时检测 (POCT) 试剂盒，用于检测促甲状腺激素 (TSH)。在将样品滴入 POCT 试剂盒后 30 分钟内即可获得测试结果。TSH 的分析结果显示出线性范围高达 55 μIU mL(-1)，在信噪比 (SNR) 为 3 时检测限 (LOD) 为 1.9 μIU mL(-1)。储存在芯片上储液器中的试剂在 120 天的储存时间内保持超过 97%的初始体积，而检测抗体在 120 天内保持超过 98%的活性。本工作中使用可大规模生产且低成本聚合物开发的样本到答案聚合物芯片实验室非常适合用于即时检测 TSH 的快速体外诊断 (IVD)。
