Crepaldi T, Richiardi P, Malavasi F, Carbonara A O, Igarashi M, Ferrone S
Tissue Antigens. 1985 Jul;26(1):25-34. doi: 10.1111/j.1399-0039.1985.tb00931.x.
2-dimensional gel electrophoresis analysis and sequential immunoprecipitation studies have shown that the monoclonal antibody (MoAb) AC1.59 recognizes a gene product of the HLA-DR locus. The determinant defined by the MoAb AC-1.59 has an unusual distribution on HLA-DR allospecificities, since it is expressed by HLA-DR1, DRw8, DRw9 antigens and by subtypes of HLA-DRw6 and HLA-DR4 antigens. In the latter allospecificity the expression of the MoAb AC1.59 defined determinant correlates with the cellularly defined HLA-Dw4 alloantigen. The present investigation provides evidence of serological heterogeneity within HLA-DR alloantigens. Furthermore these results suggest that monoclonal antibodies can sharpen the discriminatory power of serological assays to dissect the heterogeneity and complexity of HLA-DR alloantigens.
二维凝胶电泳分析和连续免疫沉淀研究表明，单克隆抗体（MoAb）AC1.59识别HLA - DR基因座的一种基因产物。由MoAb AC - 1.59定义的决定簇在HLA - DR同种特异性上具有不寻常的分布，因为它由HLA - DR1、DRw8、DRw9抗原以及HLA - DRw6和HLA - DR4抗原的亚型表达。在后者的同种特异性中，MoAb AC1.59定义的决定簇的表达与细胞定义的HLA - Dw4同种抗原相关。本研究提供了HLA - DR同种抗原内血清学异质性的证据。此外，这些结果表明单克隆抗体可以增强血清学检测的辨别能力，以剖析HLA - DR同种抗原的异质性和复杂性。
