Rombaut B, Vrijsen R, Delgadillo R, Vanden Berghe D, Boeyé A
Virology. 1985 Oct 15;146(1):111-9. doi: 10.1016/0042-6822(85)90057-1.
Using detergents, 45 S particles could be extracted from poliovirus-infected Vero and human embryonic kidney monolayer cells, but not from HeLa suspension cells. They were composed of the capsid proteins VP0, VP1, and VP3, devoid of RNA, and extremely sensitive to heat or to a slightly alkaline pH. The 45 S particles possessed neutralization epitopes of the N1 and N2 classes, as well as a VP3-linked epitope. The N2 epitopes were lost upon denaturation. In the presence of cell extracts 45 S particles were, like 14 S subunits, assembled to 71 S empty capsids expressing the N1 and N2 epitopes.
使用去污剂，可以从感染脊髓灰质炎病毒的非洲绿猴肾细胞（Vero细胞）和人胚肾单层细胞中提取出45S颗粒，但不能从海拉悬浮细胞中提取。它们由衣壳蛋白VP0、VP1和VP3组成，不含RNA，对热或微碱性pH极为敏感。45S颗粒具有N1和N2类中和表位以及一个与VP3相连的表位。N2表位在变性后丧失。在细胞提取物存在的情况下，45S颗粒像14S亚基一样，组装成表达N1和N2表位的71S空衣壳。
