Fazekas S, Fehér G, Kondics L, Ováry I, Székessy-Hermann V
Acta Physiol Hung. 1985;66(1):5-25.
The present work is concerned with the study of myosin fractions prepared from the hatching muscle (m. complexus) and a control muscle (m. pectoralis) of the developing goose embryo. The m. complexus attained its maximum mass at hatching and in the 4-day-old bird the mass of this muscle was only one fourth of that recorded at hatching. The m. complexus was hypertrophied already on the 21st day. At days 21, 27 and 28 of incubation and at posthatching days myosin preparations were made from both muscles. Partial purification of myosins from both sources yielded a high molecular weight fraction characteristic of the adult bird and one other protein fraction with molecular mass half of myosin. Both preparations exhibited the characteristic properties of myosin. The lower molecular weight fraction was also shown to develop filamentous aggregates as did the higher molecular-weight, gel filtrated myosin. The phosphate content of the half molecular mass myosin fraction prepared from the embryonic m. complexus at days prior to hatching was considerably higher than that of the high molecular weight fraction and the predominant component was P-Arg. Since the embryonic myosin was still not available in the m. complexus of the 4-day-old birds and the hypertrophied muscle underwent regression after hatching it appears that this myosin fraction is actively involved in breaking through the shell during the hatching period in geese.
本研究旨在对发育中的鹅胚胎的孵化肌（复合肌）和对照肌（胸肌）制备的肌球蛋白组分进行研究。复合肌在孵化时达到最大质量，在4日龄雏鸟中，该肌肉的质量仅为孵化时记录质量的四分之一。复合肌在第21天时就已肥大。在孵化的第21、27和28天以及孵化后各天，从这两块肌肉制备了肌球蛋白制剂。从这两种来源对肌球蛋白进行部分纯化，得到了一种成年鸟类特有的高分子量组分以及另一种分子量为肌球蛋白一半的蛋白质组分。两种制剂均表现出肌球蛋白的特征性质。较低分子量的组分也显示出与较高分子量的凝胶过滤肌球蛋白一样形成丝状聚集体。在孵化前几天从胚胎复合肌制备的分子量为肌球蛋白一半的组分的磷酸盐含量明显高于高分子量组分，且主要成分是磷酸精氨酸。由于在4日龄雏鸟的复合肌中仍未检测到胚胎肌球蛋白，并且肥大的肌肉在孵化后发生退化，因此看来这种肌球蛋白组分在鹅的孵化期积极参与破壳过程。
